Factors influencing antioxidant activities and total phenolic content of guava leaf extract
The aim of this study is to investigate the influence of certain factors on the yield, antioxidant activity (AA) and total phenolic content (TPC) of guava leaf extract. The effects of pretreatment of leaf sample prior to extract, extraction method, and the leaf age were investigated. FolineCiocaltea...
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th-cmuir.6653943832-505032018-09-04T04:41:39Z Factors influencing antioxidant activities and total phenolic content of guava leaf extract Witayapan Nantitanon Songwut Yotsawimonwat Siriporn Okonogi Agricultural and Biological Sciences The aim of this study is to investigate the influence of certain factors on the yield, antioxidant activity (AA) and total phenolic content (TPC) of guava leaf extract. The effects of pretreatment of leaf sample prior to extract, extraction method, and the leaf age were investigated. FolineCiocalteau was used to determine the TPC. Trolox equivalent antioxidant capacity (TEAC) and equilibrium concentration (EC) were used for evaluation of AA. Results indicated that ultrasonication is the most suitable method for guava leaf extraction as it yielded the extract with the significantly highest TPC and AA. Blanching followed by ice water cooling (BCD) was suggested for the pretreatment process of guava leaves. The study of leaf maturity demonstrated that the highest activity was from the young leaves. Hot water was the best solvent to extract the active principles. The extract of BCD pretreated young leaves, extracted by hot water exhibited the highest TPC and AA with the TEAC and EC values of 24.30 ± 0.50 and 20.41 ± 0.67 mM/mg, respectively. These values are 1.88 and 8.72 times higher than the synthetic antioxidant butylated hydroxy toluene (BHT) and 1.75 and 1.21 times higher than vitamin E, respectively. It was concluded that pretreatment and drying process, method of extraction and leaf maturity played important roles on the bioactive compounds and their antioxidant power of guava leaf extract. © 2010 Elsevier Ltd. 2018-09-04T04:41:39Z 2018-09-04T04:41:39Z 2010-01-01 Journal 00236438 2-s2.0-78149436244 10.1016/j.lwt.2010.02.015 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78149436244&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/50503 |
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Agricultural and Biological Sciences Witayapan Nantitanon Songwut Yotsawimonwat Siriporn Okonogi Factors influencing antioxidant activities and total phenolic content of guava leaf extract |
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The aim of this study is to investigate the influence of certain factors on the yield, antioxidant activity (AA) and total phenolic content (TPC) of guava leaf extract. The effects of pretreatment of leaf sample prior to extract, extraction method, and the leaf age were investigated. FolineCiocalteau was used to determine the TPC. Trolox equivalent antioxidant capacity (TEAC) and equilibrium concentration (EC) were used for evaluation of AA. Results indicated that ultrasonication is the most suitable method for guava leaf extraction as it yielded the extract with the significantly highest TPC and AA. Blanching followed by ice water cooling (BCD) was suggested for the pretreatment process of guava leaves. The study of leaf maturity demonstrated that the highest activity was from the young leaves. Hot water was the best solvent to extract the active principles. The extract of BCD pretreated young leaves, extracted by hot water exhibited the highest TPC and AA with the TEAC and EC values of 24.30 ± 0.50 and 20.41 ± 0.67 mM/mg, respectively. These values are 1.88 and 8.72 times higher than the synthetic antioxidant butylated hydroxy toluene (BHT) and 1.75 and 1.21 times higher than vitamin E, respectively. It was concluded that pretreatment and drying process, method of extraction and leaf maturity played important roles on the bioactive compounds and their antioxidant power of guava leaf extract. © 2010 Elsevier Ltd. |
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Witayapan Nantitanon Songwut Yotsawimonwat Siriporn Okonogi |
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Witayapan Nantitanon Songwut Yotsawimonwat Siriporn Okonogi |
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Witayapan Nantitanon |
title |
Factors influencing antioxidant activities and total phenolic content of guava leaf extract |
title_short |
Factors influencing antioxidant activities and total phenolic content of guava leaf extract |
title_full |
Factors influencing antioxidant activities and total phenolic content of guava leaf extract |
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Factors influencing antioxidant activities and total phenolic content of guava leaf extract |
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Factors influencing antioxidant activities and total phenolic content of guava leaf extract |
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factors influencing antioxidant activities and total phenolic content of guava leaf extract |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78149436244&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/50503 |
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