Effects of gamma oryzanol and proanthocyanidin from purple glutinous rice bran (Oryza sativa L.) on antibody production and oxidative status in oxidative stress BALB/c mice

Gamma oryzanol (GO) and proanthocyanidin (PA) are antioxidants of purple glutinous rice bran (Oryza sativa L.). Both antioxidants give positive effects on health as "Functional Food". The objective of this study was determined the effect of oral-fed antioxidant substances of purple glutino...

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Main Authors: Montri Punyatong, Puntipa Pongpiachan, Petai Pongpiachan, Yanee Pongpaibul, Samlee Manketkorn
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/50508
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spelling th-cmuir.6653943832-505082018-09-04T04:52:53Z Effects of gamma oryzanol and proanthocyanidin from purple glutinous rice bran (Oryza sativa L.) on antibody production and oxidative status in oxidative stress BALB/c mice Montri Punyatong Puntipa Pongpiachan Petai Pongpiachan Yanee Pongpaibul Samlee Manketkorn Agricultural and Biological Sciences Chemistry Pharmacology, Toxicology and Pharmaceutics Gamma oryzanol (GO) and proanthocyanidin (PA) are antioxidants of purple glutinous rice bran (Oryza sativa L.). Both antioxidants give positive effects on health as "Functional Food". The objective of this study was determined the effect of oral-fed antioxidant substances of purple glutinous rice (Oryza sativa L.) on antibody production and oxidative status in oxidative stress BALB/c mice. The experiment was divided into two steps. One was in vitro test: to find out the equilibrium activity between oxidant (rancid rice bran oil, RO) and antioxidants (GO and PA). Two was in vivo test: to measure the antibody value and oxidative status in BALB/c mice which intake equilibrium ratios of oxidant to antioxidants from in vitro test. The in vitro test has shown equilibrium activity between oxidant to antioxidants, RO:GO at 1:0.8 ratio (antioxidant level after reacted with oxidant = 2.514 ± 0.838 μM/mg) and RO:PA at 1:0.6 ratio (antioxidant level after reacted with oxidant = 13.768 ± 0.785 μM/mg). The in vivo test gave that the IgG and IgM level were decreased (p<0.05) when fed only RO but increased to initial normal levels when fed RO plus GO or PA. Also in glutathione (GSH) and malondialdehyde (MDA) level in blood which had related results with antibody levels (p<0.05). In conclusion, supplemented antioxidant substances of purple glutinous rice to the oxidative stress mice at equilibrium ratios could recover the antibody level and balancing of oxidative status in the body to normal status. It can be concluded that GO and PA of purple glutinous rice display the beneficial effect to the health and can used for "Functional Food" to improving the oxidative stress. 2018-09-04T04:41:42Z 2018-09-04T04:41:42Z 2010-01-01 Journal 09756892 09754261 2-s2.0-79956066716 10.5958/j.0975-4261.2.3.031 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79956066716&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/50508
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Chemistry
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Agricultural and Biological Sciences
Chemistry
Pharmacology, Toxicology and Pharmaceutics
Montri Punyatong
Puntipa Pongpiachan
Petai Pongpiachan
Yanee Pongpaibul
Samlee Manketkorn
Effects of gamma oryzanol and proanthocyanidin from purple glutinous rice bran (Oryza sativa L.) on antibody production and oxidative status in oxidative stress BALB/c mice
description Gamma oryzanol (GO) and proanthocyanidin (PA) are antioxidants of purple glutinous rice bran (Oryza sativa L.). Both antioxidants give positive effects on health as "Functional Food". The objective of this study was determined the effect of oral-fed antioxidant substances of purple glutinous rice (Oryza sativa L.) on antibody production and oxidative status in oxidative stress BALB/c mice. The experiment was divided into two steps. One was in vitro test: to find out the equilibrium activity between oxidant (rancid rice bran oil, RO) and antioxidants (GO and PA). Two was in vivo test: to measure the antibody value and oxidative status in BALB/c mice which intake equilibrium ratios of oxidant to antioxidants from in vitro test. The in vitro test has shown equilibrium activity between oxidant to antioxidants, RO:GO at 1:0.8 ratio (antioxidant level after reacted with oxidant = 2.514 ± 0.838 μM/mg) and RO:PA at 1:0.6 ratio (antioxidant level after reacted with oxidant = 13.768 ± 0.785 μM/mg). The in vivo test gave that the IgG and IgM level were decreased (p<0.05) when fed only RO but increased to initial normal levels when fed RO plus GO or PA. Also in glutathione (GSH) and malondialdehyde (MDA) level in blood which had related results with antibody levels (p<0.05). In conclusion, supplemented antioxidant substances of purple glutinous rice to the oxidative stress mice at equilibrium ratios could recover the antibody level and balancing of oxidative status in the body to normal status. It can be concluded that GO and PA of purple glutinous rice display the beneficial effect to the health and can used for "Functional Food" to improving the oxidative stress.
format Journal
author Montri Punyatong
Puntipa Pongpiachan
Petai Pongpiachan
Yanee Pongpaibul
Samlee Manketkorn
author_facet Montri Punyatong
Puntipa Pongpiachan
Petai Pongpiachan
Yanee Pongpaibul
Samlee Manketkorn
author_sort Montri Punyatong
title Effects of gamma oryzanol and proanthocyanidin from purple glutinous rice bran (Oryza sativa L.) on antibody production and oxidative status in oxidative stress BALB/c mice
title_short Effects of gamma oryzanol and proanthocyanidin from purple glutinous rice bran (Oryza sativa L.) on antibody production and oxidative status in oxidative stress BALB/c mice
title_full Effects of gamma oryzanol and proanthocyanidin from purple glutinous rice bran (Oryza sativa L.) on antibody production and oxidative status in oxidative stress BALB/c mice
title_fullStr Effects of gamma oryzanol and proanthocyanidin from purple glutinous rice bran (Oryza sativa L.) on antibody production and oxidative status in oxidative stress BALB/c mice
title_full_unstemmed Effects of gamma oryzanol and proanthocyanidin from purple glutinous rice bran (Oryza sativa L.) on antibody production and oxidative status in oxidative stress BALB/c mice
title_sort effects of gamma oryzanol and proanthocyanidin from purple glutinous rice bran (oryza sativa l.) on antibody production and oxidative status in oxidative stress balb/c mice
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79956066716&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/50508
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