Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis

Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis

A real-time fluorescence resonance energy transfer (FRET) PCR supplemented with melting curve analysis for the rapid molecular detection of Dirofilaria immitis in mosquito vectors and dog blood samples was developed. This real-time FRET PCR was based on the fluorescence melting curve analysis of a h...

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Main Authors: Tongjit Thanchomnang, Pewpan M. Intapan, Viraphong Lulitanond, Somboon Sangmaneedet, Sudchit Chungpivat, Piyanan Taweethavonsawat, Wej Choochote, Wanchai Maleewong
Format: Journal
Published: 2018
Subjects:
Immunology and Microbiology
Veterinary
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77349109960&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/50931
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Institution: Chiang Mai University
id th-cmuir.6653943832-50931
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spelling th-cmuir.6653943832-509312018-09-04T04:54:22Z Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis Tongjit Thanchomnang Pewpan M. Intapan Viraphong Lulitanond Somboon Sangmaneedet Sudchit Chungpivat Piyanan Taweethavonsawat Wej Choochote Wanchai Maleewong Immunology and Microbiology Veterinary A real-time fluorescence resonance energy transfer (FRET) PCR supplemented with melting curve analysis for the rapid molecular detection of Dirofilaria immitis in mosquito vectors and dog blood samples was developed. This real-time FRET PCR was based on the fluorescence melting curve analysis of a hybrid between an amplicon generated from the D. immitis ribosomal RNA gene sequence and specific fluorophore-labeled probes. The sensitivity, specificity, accuracy, and positive and negative predictive values of this method were all 100%. Besides being highly sensitive and specific, this PCR is fast and offers a high throughput. Therefore it is a suitable and powerful tool for the diagnosis and for epidemiological surveys of canine dirofilariasis as well as for molecular xenomonitoring of D. immitis in mosquito vectors. © 2009 Elsevier B.V. All rights reserved. 2018-09-04T04:47:43Z 2018-09-04T04:47:43Z 2010-03-25 Journal 03044017 2-s2.0-77349109960 10.1016/j.vetpar.2009.12.002 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77349109960&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/50931
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Microbiology
Veterinary
spellingShingle Immunology and Microbiology
Veterinary
Tongjit Thanchomnang
Pewpan M. Intapan
Viraphong Lulitanond
Somboon Sangmaneedet
Sudchit Chungpivat
Piyanan Taweethavonsawat
Wej Choochote
Wanchai Maleewong
Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis
description A real-time fluorescence resonance energy transfer (FRET) PCR supplemented with melting curve analysis for the rapid molecular detection of Dirofilaria immitis in mosquito vectors and dog blood samples was developed. This real-time FRET PCR was based on the fluorescence melting curve analysis of a hybrid between an amplicon generated from the D. immitis ribosomal RNA gene sequence and specific fluorophore-labeled probes. The sensitivity, specificity, accuracy, and positive and negative predictive values of this method were all 100%. Besides being highly sensitive and specific, this PCR is fast and offers a high throughput. Therefore it is a suitable and powerful tool for the diagnosis and for epidemiological surveys of canine dirofilariasis as well as for molecular xenomonitoring of D. immitis in mosquito vectors. © 2009 Elsevier B.V. All rights reserved.
format Journal
author Tongjit Thanchomnang
Pewpan M. Intapan
Viraphong Lulitanond
Somboon Sangmaneedet
Sudchit Chungpivat
Piyanan Taweethavonsawat
Wej Choochote
Wanchai Maleewong
author_facet Tongjit Thanchomnang
Pewpan M. Intapan
Viraphong Lulitanond
Somboon Sangmaneedet
Sudchit Chungpivat
Piyanan Taweethavonsawat
Wej Choochote
Wanchai Maleewong
author_sort Tongjit Thanchomnang
title Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis
title_short Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis
title_full Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis
title_fullStr Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis
title_full_unstemmed Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis
title_sort rapid detection of dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer pcr and melting curve analysis
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77349109960&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/50931
_version_ 1681423678406918144

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