Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis

• Main Authors: Tongjit Thanchomnang, Pewpan M. Intapan, Viraphong Lulitanond, Somboon Sangmaneedet, Sudchit Chungpivat, Piyanan Taweethavonsawat, Wej Choochote, Wanchai Maleewong
• Format: Journal
• Published: 2018
• Subjects: Immunology and Microbiology; Veterinary
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77349109960&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/50931
• Institution: Chiang Mai University

A real-time fluorescence resonance energy transfer (FRET) PCR supplemented with melting curve analysis for the rapid molecular detection of Dirofilaria immitis in mosquito vectors and dog blood samples was developed. This real-time FRET PCR was based on the fluorescence melting curve analysis of a h...