Biodegradation of glucosinolates in brown mustard seed meal (Brassica juncea) by Aspergillus sp. NR-4201 in liquid and solid-state cultures

Aspergillus sp. NR-4201 was assessed by degrading glucosinolates in brown mustard seed meal (Brassicajuncea). A liquid culture of the strain, in a medium derived from the meal, produced total degradation of glucosinolates at 32 h. Under these conditions, the glucosinolate-breakdown product, allylcya...

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Main Authors: Rakariyatham N., Sakorn P.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-0036965846&partnerID=40&md5=0665580737676e4942f73ca620326f8d
http://www.ncbi.nlm.nih.gov/pubmed/12713131
http://cmuir.cmu.ac.th/handle/6653943832/5187
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-51872014-08-30T02:56:15Z Biodegradation of glucosinolates in brown mustard seed meal (Brassica juncea) by Aspergillus sp. NR-4201 in liquid and solid-state cultures Rakariyatham N. Sakorn P. Aspergillus sp. NR-4201 was assessed by degrading glucosinolates in brown mustard seed meal (Brassicajuncea). A liquid culture of the strain, in a medium derived from the meal, produced total degradation of glucosinolates at 32 h. Under these conditions, the glucosinolate-breakdown product, allylcyanide, was formed in culture filtrates. In a plate culture under sterile conditions, the growth of the strain in heat-treated meal media was shown to be effective at 30 °C with 51% moisture, as determined by the measurement of the colony growth rate. On the laboratory scale, solid-state culture under the same conditions gave rise to total glucosinolate degradation within 48 h. In comparison, under non-sterile conditions in either heat-treated or non heat-treated meal samples, the degradations were complete after 60 and 96 h, respectively. In these cases, growth was associated with some out-growths of contaminating fungi, mainly Rhizopus sp. and Mucor sp. The glucosinolate-breakdown product, allylcyanide, was not detected in the solid-state meal-media culture presumably due to evaporative loss from the fermentation matrix. 2014-08-30T02:56:15Z 2014-08-30T02:56:15Z 2002 Article 09239820 10.1023/A:1022851129684 12713131 BIODE http://www.scopus.com/inward/record.url?eid=2-s2.0-0036965846&partnerID=40&md5=0665580737676e4942f73ca620326f8d http://www.ncbi.nlm.nih.gov/pubmed/12713131 http://cmuir.cmu.ac.th/handle/6653943832/5187 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Aspergillus sp. NR-4201 was assessed by degrading glucosinolates in brown mustard seed meal (Brassicajuncea). A liquid culture of the strain, in a medium derived from the meal, produced total degradation of glucosinolates at 32 h. Under these conditions, the glucosinolate-breakdown product, allylcyanide, was formed in culture filtrates. In a plate culture under sterile conditions, the growth of the strain in heat-treated meal media was shown to be effective at 30 °C with 51% moisture, as determined by the measurement of the colony growth rate. On the laboratory scale, solid-state culture under the same conditions gave rise to total glucosinolate degradation within 48 h. In comparison, under non-sterile conditions in either heat-treated or non heat-treated meal samples, the degradations were complete after 60 and 96 h, respectively. In these cases, growth was associated with some out-growths of contaminating fungi, mainly Rhizopus sp. and Mucor sp. The glucosinolate-breakdown product, allylcyanide, was not detected in the solid-state meal-media culture presumably due to evaporative loss from the fermentation matrix.
format Article
author Rakariyatham N.
Sakorn P.
spellingShingle Rakariyatham N.
Sakorn P.
Biodegradation of glucosinolates in brown mustard seed meal (Brassica juncea) by Aspergillus sp. NR-4201 in liquid and solid-state cultures
author_facet Rakariyatham N.
Sakorn P.
author_sort Rakariyatham N.
title Biodegradation of glucosinolates in brown mustard seed meal (Brassica juncea) by Aspergillus sp. NR-4201 in liquid and solid-state cultures
title_short Biodegradation of glucosinolates in brown mustard seed meal (Brassica juncea) by Aspergillus sp. NR-4201 in liquid and solid-state cultures
title_full Biodegradation of glucosinolates in brown mustard seed meal (Brassica juncea) by Aspergillus sp. NR-4201 in liquid and solid-state cultures
title_fullStr Biodegradation of glucosinolates in brown mustard seed meal (Brassica juncea) by Aspergillus sp. NR-4201 in liquid and solid-state cultures
title_full_unstemmed Biodegradation of glucosinolates in brown mustard seed meal (Brassica juncea) by Aspergillus sp. NR-4201 in liquid and solid-state cultures
title_sort biodegradation of glucosinolates in brown mustard seed meal (brassica juncea) by aspergillus sp. nr-4201 in liquid and solid-state cultures
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-0036965846&partnerID=40&md5=0665580737676e4942f73ca620326f8d
http://www.ncbi.nlm.nih.gov/pubmed/12713131
http://cmuir.cmu.ac.th/handle/6653943832/5187
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