A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model

Coomassie brilliant blue (CBB) heated by microwave was used as a staining dye for measuring gelatinolytic activity. The quantity of gelatin remaining after incubation with bacterial collagenase was determined using the heated CBB, resulting in visible blue pellets. Dimethyl sulfoxide was added to di...

Full description

Saved in:

Bibliographic Details
Main Authors:	Maslin Osathanunkul, Kittisak Buddhachat, Siriwadee Chomdej
Format:	Journal
Published:	2018
Subjects:	Biochemistry, Genetics and Molecular Biology
Online Access:	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84870695160&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/52270
Tags:	Add Tag No Tags, Be the first to tag this record!
Institution:	Chiang Mai University

id	th-cmuir.6653943832-52270
record_format	dspace
spelling	th-cmuir.6653943832-522702018-09-04T09:22:55Z A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model Maslin Osathanunkul Kittisak Buddhachat Siriwadee Chomdej Biochemistry, Genetics and Molecular Biology Coomassie brilliant blue (CBB) heated by microwave was used as a staining dye for measuring gelatinolytic activity. The quantity of gelatin remaining after incubation with bacterial collagenase was determined using the heated CBB, resulting in visible blue pellets. Dimethyl sulfoxide was added to dissolve the dye and measurement of the absorbance at 600 nm was done to detect the level of gelatin (up to 10 μg), with the limit of detection for the amount of collagenase at 50 ng. This approach is rapid, simple, and economic for the purpose of screening for pharmaceutical agents that possess inhibitory activity on collagenase. © 2012 Elsevier Inc. All rights reserved. 2018-09-04T09:22:55Z 2018-09-04T09:22:55Z 2013-02-15 Journal 10960309 00032697 2-s2.0-84870695160 10.1016/j.ab.2012.09.036 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84870695160&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/52270
institution	Chiang Mai University
building	Chiang Mai University Library
country	Thailand
collection	CMU Intellectual Repository
topic	Biochemistry, Genetics and Molecular Biology
spellingShingle	Biochemistry, Genetics and Molecular Biology Maslin Osathanunkul Kittisak Buddhachat Siriwadee Chomdej A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
description	Coomassie brilliant blue (CBB) heated by microwave was used as a staining dye for measuring gelatinolytic activity. The quantity of gelatin remaining after incubation with bacterial collagenase was determined using the heated CBB, resulting in visible blue pellets. Dimethyl sulfoxide was added to dissolve the dye and measurement of the absorbance at 600 nm was done to detect the level of gelatin (up to 10 μg), with the limit of detection for the amount of collagenase at 50 ng. This approach is rapid, simple, and economic for the purpose of screening for pharmaceutical agents that possess inhibitory activity on collagenase. © 2012 Elsevier Inc. All rights reserved.
format	Journal
author	Maslin Osathanunkul Kittisak Buddhachat Siriwadee Chomdej
author_facet	Maslin Osathanunkul Kittisak Buddhachat Siriwadee Chomdej
author_sort	Maslin Osathanunkul
title	A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
title_short	A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
title_full	A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
title_fullStr	A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
title_full_unstemmed	A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
title_sort	modified colorimetric method of gelatinolytic assay using bacterial collagenase type ii as a model
publishDate	2018
url	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84870695160&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/52270
_version_	1681423920557719552

A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model

Similar Items