Sensitive, simultaneous quantitation of two unlabeled DNA targets using a magnetic nanoparticle-enzyme sandwich assay

• المؤلفون الرئيسيون: Yue Zhang, Chalermchai Pilapong, Yuan Guo, Zhenlian Ling, Oscar Cespedes, Philip Quirke, Dejian Zhou
• التنسيق: دورية
• منشور في: 2018
• الموضوعات: Chemistry
• الوصول للمادة أونلاين: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84884996190&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/52373

We report herein the development of a simple, sensitive colorimetric magnetic nanoparticle (MNP)-enzyme-based DNA sandwich assay that is suitable for simultaneous, label-free quantitation of two DNA targets down to 50 fM level. It can also effectively discriminate single-nucleotide polymorphisms (SN...