The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand

Hyperthermophilic community diversity was assessed in hot-spring streamers along gradients of temperature, pH and sulphide in northern Thailand. A hierarchical sampling design was employed to obtain biomass for culture-independent estimates of 16S rRNA gene-defined prokaryotic diversity. All springs...

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Main Authors: Purcell D., Sompong U., Yim L.C., Barraclough T.G., Peerapornpisal Y., Pointing S.B.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-34249095755&partnerID=40&md5=551a1fec37a61de06675920006577922
http://cmuir.cmu.ac.th/handle/6653943832/5251
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-52512014-08-30T02:56:19Z The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand Purcell D. Sompong U. Yim L.C. Barraclough T.G. Peerapornpisal Y. Pointing S.B. Hyperthermophilic community diversity was assessed in hot-spring streamers along gradients of temperature, pH and sulphide in northern Thailand. A hierarchical sampling design was employed to obtain biomass for culture-independent estimates of 16S rRNA gene-defined prokaryotic diversity. All springs supported several archaeal and bacterial phylotypes, including novel phylotypes that expand the known phylogenetic diversity of terrestrial hyperthermophiles. Diversity appeared significantly greater than that observed for several other geographic locations. Phylotypes belonging to the Aquificales were ubiquitous, further supporting the hypothesis that these chemolithoautotrophs are key members of all hyperthermophilic communities. The chemoorganotrophic genus Thermus was also represented by phylotypes in all springs. Other bacterial taxa represented by environmental sequences included Bacillus, Thermotoga and various unidentified Alphaproteobacteria and Betaproteobacteria. Archaeal phylotypes included the Crenarchaea Desulfurococcus, Pyrobaculum, plus several unidentified hyperthermophilic lineages. A Methanothermococcus-like Euryarchaeon was also identified, with this genus not previously known from streamer communities. A multivariate approach to the analysis of biotic and abiotic data revealed that diversity patterns were best explained by a combination of temperature and sulphide rather than by any other abiotic variable either individually or in combination. © 2007 Federation of European Microbiological Societies. 2014-08-30T02:56:19Z 2014-08-30T02:56:19Z 2007 Article 01686496 10.1111/j.1574-6941.2007.00302.x 17386034 FMECE http://www.scopus.com/inward/record.url?eid=2-s2.0-34249095755&partnerID=40&md5=551a1fec37a61de06675920006577922 http://cmuir.cmu.ac.th/handle/6653943832/5251 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Hyperthermophilic community diversity was assessed in hot-spring streamers along gradients of temperature, pH and sulphide in northern Thailand. A hierarchical sampling design was employed to obtain biomass for culture-independent estimates of 16S rRNA gene-defined prokaryotic diversity. All springs supported several archaeal and bacterial phylotypes, including novel phylotypes that expand the known phylogenetic diversity of terrestrial hyperthermophiles. Diversity appeared significantly greater than that observed for several other geographic locations. Phylotypes belonging to the Aquificales were ubiquitous, further supporting the hypothesis that these chemolithoautotrophs are key members of all hyperthermophilic communities. The chemoorganotrophic genus Thermus was also represented by phylotypes in all springs. Other bacterial taxa represented by environmental sequences included Bacillus, Thermotoga and various unidentified Alphaproteobacteria and Betaproteobacteria. Archaeal phylotypes included the Crenarchaea Desulfurococcus, Pyrobaculum, plus several unidentified hyperthermophilic lineages. A Methanothermococcus-like Euryarchaeon was also identified, with this genus not previously known from streamer communities. A multivariate approach to the analysis of biotic and abiotic data revealed that diversity patterns were best explained by a combination of temperature and sulphide rather than by any other abiotic variable either individually or in combination. © 2007 Federation of European Microbiological Societies.
format Article
author Purcell D.
Sompong U.
Yim L.C.
Barraclough T.G.
Peerapornpisal Y.
Pointing S.B.
spellingShingle Purcell D.
Sompong U.
Yim L.C.
Barraclough T.G.
Peerapornpisal Y.
Pointing S.B.
The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand
author_facet Purcell D.
Sompong U.
Yim L.C.
Barraclough T.G.
Peerapornpisal Y.
Pointing S.B.
author_sort Purcell D.
title The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand
title_short The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand
title_full The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand
title_fullStr The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand
title_full_unstemmed The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand
title_sort effects of temperature, ph and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern thailand
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-34249095755&partnerID=40&md5=551a1fec37a61de06675920006577922
http://cmuir.cmu.ac.th/handle/6653943832/5251
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