Response surface optimization of exopolysaccharide production from sugarcane juice by Lactobacillus confusus TISTR 1498

Lactobacillus confusus TISTR 1498, isolated from Thai traditional fermented pork (Nham), could secrete large amounts of exopolysaccharides (EPS). Response surface methodology was applied to optimize the culture conditions for EPS production using Central Composite Design (CCD). The effects of three...

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Main Authors: Phayungsak Manochai, Yuthana Phimolsiripol, Phisit Seesuriyachan
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/53944
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-539442018-09-04T10:03:11Z Response surface optimization of exopolysaccharide production from sugarcane juice by Lactobacillus confusus TISTR 1498 Phayungsak Manochai Yuthana Phimolsiripol Phisit Seesuriyachan Multidisciplinary Lactobacillus confusus TISTR 1498, isolated from Thai traditional fermented pork (Nham), could secrete large amounts of exopolysaccharides (EPS). Response surface methodology was applied to optimize the culture conditions for EPS production using Central Composite Design (CCD). The effects of three variables including pH (4-7), temperature (20-40°C) and nitrogen source (0.25-1.75 PYB) on EPS yield and biomass were investigated. The 1PYB was the mixtures of 5 g/L peptone (P), 2.5 g/L yeast extract (Y) and 2.5 g/L beef extract (B). Response surface methodology showed that the data were adequately fitted to a second-order polynomial model via quadratic regression relationships. The optimal culture conditions for EPS production in sugarcane juice were pH of 5.55, 29.75°C and 1.22PYB, which was composed of 6.1 g/L peptone, 3.05 g/L yeast extract and 3.05 g/L beef extract. Under the optimum condition, the predicted maximum EPS production was 107.5 g/L and the predicted biomass was 1.92 g/L. In submerged fermentation, sugarcane juice enhanced EPS yield twice as well as the modified MRS sucrose medium. In addition, the cost of medium can be lowered to 0.53 THB/g EPS, which was lower than that of the medium from the mixtures of the PYB (0.78 THB/gEPS). 2018-09-04T10:03:11Z 2018-09-04T10:03:11Z 2014-01-01 Journal 16851994 2-s2.0-84937542235 10.12982/cmujns.2014.0046 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84937542235&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/53944
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Multidisciplinary
spellingShingle Multidisciplinary
Phayungsak Manochai
Yuthana Phimolsiripol
Phisit Seesuriyachan
Response surface optimization of exopolysaccharide production from sugarcane juice by Lactobacillus confusus TISTR 1498
description Lactobacillus confusus TISTR 1498, isolated from Thai traditional fermented pork (Nham), could secrete large amounts of exopolysaccharides (EPS). Response surface methodology was applied to optimize the culture conditions for EPS production using Central Composite Design (CCD). The effects of three variables including pH (4-7), temperature (20-40°C) and nitrogen source (0.25-1.75 PYB) on EPS yield and biomass were investigated. The 1PYB was the mixtures of 5 g/L peptone (P), 2.5 g/L yeast extract (Y) and 2.5 g/L beef extract (B). Response surface methodology showed that the data were adequately fitted to a second-order polynomial model via quadratic regression relationships. The optimal culture conditions for EPS production in sugarcane juice were pH of 5.55, 29.75°C and 1.22PYB, which was composed of 6.1 g/L peptone, 3.05 g/L yeast extract and 3.05 g/L beef extract. Under the optimum condition, the predicted maximum EPS production was 107.5 g/L and the predicted biomass was 1.92 g/L. In submerged fermentation, sugarcane juice enhanced EPS yield twice as well as the modified MRS sucrose medium. In addition, the cost of medium can be lowered to 0.53 THB/g EPS, which was lower than that of the medium from the mixtures of the PYB (0.78 THB/gEPS).
format Journal
author Phayungsak Manochai
Yuthana Phimolsiripol
Phisit Seesuriyachan
author_facet Phayungsak Manochai
Yuthana Phimolsiripol
Phisit Seesuriyachan
author_sort Phayungsak Manochai
title Response surface optimization of exopolysaccharide production from sugarcane juice by Lactobacillus confusus TISTR 1498
title_short Response surface optimization of exopolysaccharide production from sugarcane juice by Lactobacillus confusus TISTR 1498
title_full Response surface optimization of exopolysaccharide production from sugarcane juice by Lactobacillus confusus TISTR 1498
title_fullStr Response surface optimization of exopolysaccharide production from sugarcane juice by Lactobacillus confusus TISTR 1498
title_full_unstemmed Response surface optimization of exopolysaccharide production from sugarcane juice by Lactobacillus confusus TISTR 1498
title_sort response surface optimization of exopolysaccharide production from sugarcane juice by lactobacillus confusus tistr 1498
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84937542235&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/53944
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