Isolation and screening of biopolymer-degrading microorganisms from northern Thailand
© 2015, Springer Science+Business Media Dordrecht. Forty agricultural soils were collected from Chiang Mai and Lampang provinces in northern Thailand. Bacteria, actinomycetes and fungi were isolated and screened for their ability to degrade polylactic acid (PLA), polycaprolactone (PCL) and poly(buty...
Saved in:
Main Authors: | , , , , , |
---|---|
Format: | Journal |
Published: |
2018
|
Subjects: | |
Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84938747937&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/54121 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Chiang Mai University |
id |
th-cmuir.6653943832-54121 |
---|---|
record_format |
dspace |
spelling |
th-cmuir.6653943832-541212018-09-04T10:16:41Z Isolation and screening of biopolymer-degrading microorganisms from northern Thailand Watsana Penkhrue Chartchai Khanongnuch Kazuo Masaki Wasu Pathom-aree Winita Punyodom Saisamorn Lumyong Biochemistry, Genetics and Molecular Biology Immunology and Microbiology © 2015, Springer Science+Business Media Dordrecht. Forty agricultural soils were collected from Chiang Mai and Lampang provinces in northern Thailand. Bacteria, actinomycetes and fungi were isolated and screened for their ability to degrade polylactic acid (PLA), polycaprolactone (PCL) and poly(butylene succinate) (PBS) by the agar diffusion method. Sixty-seven actinomycetes, seven bacteria and five fungal isolates were obtained. The majority of actinomycetes were Streptomyces based on morphological characteristic, chemotaxonomy and 16S rRNA gene data. Seventy-nine microorganisms were isolated from 40 soil samples. Twenty-six isolates showed PLA-degradation (32.9 %), 44 isolates showed PBS-degradation (55.7 %) and 58 isolates showed PCL-degradation (73.4 %). Interestingly, 16 isolates (20.2 %) could degrade all three types of bioplastics used in this study. The Amycolatopsis sp. strain SCM_MK2-4 showed the highest enzyme activity for both PLA and PCL, 0.046 and 0.023 U/mL, respectively. Moreover, this strain produced protease, esterase and lipase on agar plates. Approximately, 36.7 % of the PLA film was degraded by Amycolatopsis sp. SCM_MK2-4 after 7 days of cultivation at 30 °C in culture broth. 2018-09-04T10:07:58Z 2018-09-04T10:07:58Z 2015-09-10 Journal 15730972 09593993 2-s2.0-84938747937 10.1007/s11274-015-1895-1 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84938747937&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/54121 |
institution |
Chiang Mai University |
building |
Chiang Mai University Library |
country |
Thailand |
collection |
CMU Intellectual Repository |
topic |
Biochemistry, Genetics and Molecular Biology Immunology and Microbiology |
spellingShingle |
Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Watsana Penkhrue Chartchai Khanongnuch Kazuo Masaki Wasu Pathom-aree Winita Punyodom Saisamorn Lumyong Isolation and screening of biopolymer-degrading microorganisms from northern Thailand |
description |
© 2015, Springer Science+Business Media Dordrecht. Forty agricultural soils were collected from Chiang Mai and Lampang provinces in northern Thailand. Bacteria, actinomycetes and fungi were isolated and screened for their ability to degrade polylactic acid (PLA), polycaprolactone (PCL) and poly(butylene succinate) (PBS) by the agar diffusion method. Sixty-seven actinomycetes, seven bacteria and five fungal isolates were obtained. The majority of actinomycetes were Streptomyces based on morphological characteristic, chemotaxonomy and 16S rRNA gene data. Seventy-nine microorganisms were isolated from 40 soil samples. Twenty-six isolates showed PLA-degradation (32.9 %), 44 isolates showed PBS-degradation (55.7 %) and 58 isolates showed PCL-degradation (73.4 %). Interestingly, 16 isolates (20.2 %) could degrade all three types of bioplastics used in this study. The Amycolatopsis sp. strain SCM_MK2-4 showed the highest enzyme activity for both PLA and PCL, 0.046 and 0.023 U/mL, respectively. Moreover, this strain produced protease, esterase and lipase on agar plates. Approximately, 36.7 % of the PLA film was degraded by Amycolatopsis sp. SCM_MK2-4 after 7 days of cultivation at 30 °C in culture broth. |
format |
Journal |
author |
Watsana Penkhrue Chartchai Khanongnuch Kazuo Masaki Wasu Pathom-aree Winita Punyodom Saisamorn Lumyong |
author_facet |
Watsana Penkhrue Chartchai Khanongnuch Kazuo Masaki Wasu Pathom-aree Winita Punyodom Saisamorn Lumyong |
author_sort |
Watsana Penkhrue |
title |
Isolation and screening of biopolymer-degrading microorganisms from northern Thailand |
title_short |
Isolation and screening of biopolymer-degrading microorganisms from northern Thailand |
title_full |
Isolation and screening of biopolymer-degrading microorganisms from northern Thailand |
title_fullStr |
Isolation and screening of biopolymer-degrading microorganisms from northern Thailand |
title_full_unstemmed |
Isolation and screening of biopolymer-degrading microorganisms from northern Thailand |
title_sort |
isolation and screening of biopolymer-degrading microorganisms from northern thailand |
publishDate |
2018 |
url |
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84938747937&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/54121 |
_version_ |
1681424262096748544 |