Effects of Iron Chelators on Pulmonary Iron Overload and Oxidative Stress in β-Thalassemic Mice

© 2015 S. Karger AG, Basel. Aim: To evaluate the effect of iron chelators on iron-related pulmonary pathology and oxidative stress in an animal model of β-thalassemia. Methods: Pulmonary iron overload was induced in heterozygous β-globin knockout mice (muβth-3/+, BKO). Over a period of 2 weeks, 180...

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Main Authors: Paranee Yatmark, Noppawan Phumala Morales, Urai Chaisri, Surasak Wichaiyo, Warinkarn Hemstapat, Somdet Srichairatanakool, Saovaros Svasti, Suthat Fucharoen
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/54838
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spelling th-cmuir.6653943832-548382018-09-04T10:24:50Z Effects of Iron Chelators on Pulmonary Iron Overload and Oxidative Stress in β-Thalassemic Mice Paranee Yatmark Noppawan Phumala Morales Urai Chaisri Surasak Wichaiyo Warinkarn Hemstapat Somdet Srichairatanakool Saovaros Svasti Suthat Fucharoen Pharmacology, Toxicology and Pharmaceutics © 2015 S. Karger AG, Basel. Aim: To evaluate the effect of iron chelators on iron-related pulmonary pathology and oxidative stress in an animal model of β-thalassemia. Methods: Pulmonary iron overload was induced in heterozygous β-globin knockout mice (muβth-3/+, BKO). Over a period of 2 weeks, 180 mg of iron/mouse was loaded by intraperitoneal injection of iron dextran, and subsequently treated daily via intraperitoneal with either deferoxamine (DF) or deferiprone (L1) at an equimolar concentration of iron binding (0.2 and 0.6 μmol/g body weight, respectively) for 7 days. Results: Iron loading resulted in iron deposition in peribronchial regions, septa and also in alveolar macrophages with a grading score of 3. This iron burden resulted in lung epithelial injuries, fibrosis and corresponded with increased lipid peroxidation and decreased tissue catalase activity. Treatment with DF or L1 resulted in a reduction of iron-laden alveolar macrophages and decreased oxidative stress and tissue damage, showing the iron mobilizing ability of both compounds. Conclusion: Iron chelation therapy, with DF and L1, may protect against pulmonary damage by sequestering catalytic iron and improving oxidative status. It may be beneficial in the prevention of pulmonary complications in thalassemia. 2018-09-04T10:24:50Z 2018-09-04T10:24:50Z 2015-09-08 Journal 14230313 00317012 2-s2.0-84940751400 10.1159/000438994 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84940751400&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/54838
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Pharmacology, Toxicology and Pharmaceutics
spellingShingle Pharmacology, Toxicology and Pharmaceutics
Paranee Yatmark
Noppawan Phumala Morales
Urai Chaisri
Surasak Wichaiyo
Warinkarn Hemstapat
Somdet Srichairatanakool
Saovaros Svasti
Suthat Fucharoen
Effects of Iron Chelators on Pulmonary Iron Overload and Oxidative Stress in β-Thalassemic Mice
description © 2015 S. Karger AG, Basel. Aim: To evaluate the effect of iron chelators on iron-related pulmonary pathology and oxidative stress in an animal model of β-thalassemia. Methods: Pulmonary iron overload was induced in heterozygous β-globin knockout mice (muβth-3/+, BKO). Over a period of 2 weeks, 180 mg of iron/mouse was loaded by intraperitoneal injection of iron dextran, and subsequently treated daily via intraperitoneal with either deferoxamine (DF) or deferiprone (L1) at an equimolar concentration of iron binding (0.2 and 0.6 μmol/g body weight, respectively) for 7 days. Results: Iron loading resulted in iron deposition in peribronchial regions, septa and also in alveolar macrophages with a grading score of 3. This iron burden resulted in lung epithelial injuries, fibrosis and corresponded with increased lipid peroxidation and decreased tissue catalase activity. Treatment with DF or L1 resulted in a reduction of iron-laden alveolar macrophages and decreased oxidative stress and tissue damage, showing the iron mobilizing ability of both compounds. Conclusion: Iron chelation therapy, with DF and L1, may protect against pulmonary damage by sequestering catalytic iron and improving oxidative status. It may be beneficial in the prevention of pulmonary complications in thalassemia.
format Journal
author Paranee Yatmark
Noppawan Phumala Morales
Urai Chaisri
Surasak Wichaiyo
Warinkarn Hemstapat
Somdet Srichairatanakool
Saovaros Svasti
Suthat Fucharoen
author_facet Paranee Yatmark
Noppawan Phumala Morales
Urai Chaisri
Surasak Wichaiyo
Warinkarn Hemstapat
Somdet Srichairatanakool
Saovaros Svasti
Suthat Fucharoen
author_sort Paranee Yatmark
title Effects of Iron Chelators on Pulmonary Iron Overload and Oxidative Stress in β-Thalassemic Mice
title_short Effects of Iron Chelators on Pulmonary Iron Overload and Oxidative Stress in β-Thalassemic Mice
title_full Effects of Iron Chelators on Pulmonary Iron Overload and Oxidative Stress in β-Thalassemic Mice
title_fullStr Effects of Iron Chelators on Pulmonary Iron Overload and Oxidative Stress in β-Thalassemic Mice
title_full_unstemmed Effects of Iron Chelators on Pulmonary Iron Overload and Oxidative Stress in β-Thalassemic Mice
title_sort effects of iron chelators on pulmonary iron overload and oxidative stress in β-thalassemic mice
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84940751400&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/54838
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