Low-energy plasma immersion ion implantation to induce DNA transfer into bacterial E. coli

Low-energy plasma immersion ion implantation to induce DNA transfer into bacterial E. coli

© 2015 Elsevier B.V. Plasma immersion ion implantation (PIII) at low energy was for the first time applied as a novel biotechnology to induce DNA transfer into bacterial cells. Argon or nitrogen PIII at low bias voltages of 2.5, 5 and 10 kV and fluences ranging from 1 × 1012to 1 × 1017ions/cm2treate...

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Main Authors: K. Sangwijit, L. D. Yu, S. Sarapirom, S. Pitakrattananukool, S. Anuntalabhochai
Format: Journal
Published: 2018
Subjects:
Physics and Astronomy
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84948575625&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/54858
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-548582018-09-04T10:25:26Z Low-energy plasma immersion ion implantation to induce DNA transfer into bacterial E. coli K. Sangwijit L. D. Yu S. Sarapirom S. Pitakrattananukool S. Anuntalabhochai Physics and Astronomy © 2015 Elsevier B.V. Plasma immersion ion implantation (PIII) at low energy was for the first time applied as a novel biotechnology to induce DNA transfer into bacterial cells. Argon or nitrogen PIII at low bias voltages of 2.5, 5 and 10 kV and fluences ranging from 1 × 1012to 1 × 1017ions/cm2treated cells of Escherichia coli (E. coli). Subsequently, DNA transfer was operated by mixing the PIII-treated cells with DNA. Successes in PIII-induced DNA transfer were demonstrated by marker gene expressions. The induction of DNA transfer was ion-energy, fluence and DNA-size dependent. The DNA transferred in the cells was confirmed functioning. Mechanisms of the PIII-induced DNA transfer were investigated and discussed in terms of the E. coli cell envelope anatomy. Compared with conventional ion-beam-induced DNA transfer, PIII-induced DNA transfer was simpler with lower cost but higher efficiency. 2018-09-04T10:25:26Z 2018-09-04T10:25:26Z 2015-12-15 Journal 0168583X 2-s2.0-84948575625 10.1016/j.nimb.2015.07.022 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84948575625&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/54858
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Physics and Astronomy
spellingShingle Physics and Astronomy
K. Sangwijit
L. D. Yu
S. Sarapirom
S. Pitakrattananukool
S. Anuntalabhochai
Low-energy plasma immersion ion implantation to induce DNA transfer into bacterial E. coli
description © 2015 Elsevier B.V. Plasma immersion ion implantation (PIII) at low energy was for the first time applied as a novel biotechnology to induce DNA transfer into bacterial cells. Argon or nitrogen PIII at low bias voltages of 2.5, 5 and 10 kV and fluences ranging from 1 × 1012to 1 × 1017ions/cm2treated cells of Escherichia coli (E. coli). Subsequently, DNA transfer was operated by mixing the PIII-treated cells with DNA. Successes in PIII-induced DNA transfer were demonstrated by marker gene expressions. The induction of DNA transfer was ion-energy, fluence and DNA-size dependent. The DNA transferred in the cells was confirmed functioning. Mechanisms of the PIII-induced DNA transfer were investigated and discussed in terms of the E. coli cell envelope anatomy. Compared with conventional ion-beam-induced DNA transfer, PIII-induced DNA transfer was simpler with lower cost but higher efficiency.
format Journal
author K. Sangwijit
L. D. Yu
S. Sarapirom
S. Pitakrattananukool
S. Anuntalabhochai
author_facet K. Sangwijit
L. D. Yu
S. Sarapirom
S. Pitakrattananukool
S. Anuntalabhochai
author_sort K. Sangwijit
title Low-energy plasma immersion ion implantation to induce DNA transfer into bacterial E. coli
title_short Low-energy plasma immersion ion implantation to induce DNA transfer into bacterial E. coli
title_full Low-energy plasma immersion ion implantation to induce DNA transfer into bacterial E. coli
title_fullStr Low-energy plasma immersion ion implantation to induce DNA transfer into bacterial E. coli
title_full_unstemmed Low-energy plasma immersion ion implantation to induce DNA transfer into bacterial E. coli
title_sort low-energy plasma immersion ion implantation to induce dna transfer into bacterial e. coli
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84948575625&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/54858
_version_ 1681424398296285184

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