Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus

© 2016 Elsevier B.V. Treatment of chronic hepatitis B virus (HBV) infection with lamivudine-monotherapy rapidly selects mutant variants in a high proportion of individuals. Monitoring lamivudine resistance by consensus sequencing is costly and insensitive for detection of minority variants. An oligo...

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Main Authors:	Ingrid A. Beck, Rachel Payant, Nicole Ngo-Giang-Huong, Woottichai Khamduang, Laddawan Laomanit, Gonzague Jourdain, Lisa M. Frenkel
Format:	Journal
Published:	2018
Subjects:	Immunology and Microbiology
Online Access:	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84962283790&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/55894
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Institution:	Chiang Mai University

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spelling	th-cmuir.6653943832-558942018-09-05T03:03:24Z Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus Ingrid A. Beck Rachel Payant Nicole Ngo-Giang-Huong Woottichai Khamduang Laddawan Laomanit Gonzague Jourdain Lisa M. Frenkel Immunology and Microbiology © 2016 Elsevier B.V. Treatment of chronic hepatitis B virus (HBV) infection with lamivudine-monotherapy rapidly selects mutant variants in a high proportion of individuals. Monitoring lamivudine resistance by consensus sequencing is costly and insensitive for detection of minority variants. An oligonucleotide ligation assay (. OLA) for HBV lamivudine-resistance was developed and compared to consensus sequencing. Both assays detected drug resistance mutations in 35/64 (54.7%) specimens evaluated, and OLA detected minority mutants in an additional six (9.4%). OLA may offer a sensitive and inexpensive alternative to consensus sequencing for detection of HBV drug resistance in resource-limited settings. 2018-09-05T03:03:24Z 2018-09-05T03:03:24Z 2016-07-01 Journal 18790984 01660934 2-s2.0-84962283790 10.1016/j.jviromet.2016.03.014 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84962283790&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/55894
institution	Chiang Mai University
building	Chiang Mai University Library
country	Thailand
collection	CMU Intellectual Repository
topic	Immunology and Microbiology
spellingShingle	Immunology and Microbiology Ingrid A. Beck Rachel Payant Nicole Ngo-Giang-Huong Woottichai Khamduang Laddawan Laomanit Gonzague Jourdain Lisa M. Frenkel Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus
description	© 2016 Elsevier B.V. Treatment of chronic hepatitis B virus (HBV) infection with lamivudine-monotherapy rapidly selects mutant variants in a high proportion of individuals. Monitoring lamivudine resistance by consensus sequencing is costly and insensitive for detection of minority variants. An oligonucleotide ligation assay (. OLA) for HBV lamivudine-resistance was developed and compared to consensus sequencing. Both assays detected drug resistance mutations in 35/64 (54.7%) specimens evaluated, and OLA detected minority mutants in an additional six (9.4%). OLA may offer a sensitive and inexpensive alternative to consensus sequencing for detection of HBV drug resistance in resource-limited settings.
format	Journal
author	Ingrid A. Beck Rachel Payant Nicole Ngo-Giang-Huong Woottichai Khamduang Laddawan Laomanit Gonzague Jourdain Lisa M. Frenkel
author_facet	Ingrid A. Beck Rachel Payant Nicole Ngo-Giang-Huong Woottichai Khamduang Laddawan Laomanit Gonzague Jourdain Lisa M. Frenkel
author_sort	Ingrid A. Beck
title	Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus
title_short	Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus
title_full	Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus
title_fullStr	Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus
title_full_unstemmed	Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus
title_sort	development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis b virus
publishDate	2018
url	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84962283790&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/55894
_version_	1681424590574714880

Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus

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