Production of cellulase-free xylanase by a thermotolerant Streptomyces sp. grown on agricultural waste and media optimization using mixture design and Plackett-Burman experimental design methods
Cellulase-free xylanase was produced by Streptomyces sp. Ab106 on finely ground cane bagasse at 55°C. The optimal medium composition was developed by applying the mixture design and linear mathematical program, and evaluated using the Plackett-Burman experimental design. The best composition of basa...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
2014
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| Online Access: | http://www.scopus.com/inward/record.url?eid=2-s2.0-0036735527&partnerID=40&md5=60a461ba95af5b04d5f248f85186dbad http://cmuir.cmu.ac.th/handle/6653943832/578 |
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| Institution: | Chiang Mai University |
| Language: | English |
| Summary: | Cellulase-free xylanase was produced by Streptomyces sp. Ab106 on finely ground cane bagasse at 55°C. The optimal medium composition was developed by applying the mixture design and linear mathematical program, and evaluated using the Plackett-Burman experimental design. The best composition of basal medium was found by using the mixture design method. The highest xylanase activity, 10.6 IU, was obtained after 6 days of fermentation in shaked flask at 100 rpm, 55°C, pH 7. Both experimental designs showed that trace elements induced xylanase production. With fermentation in a 5-1 fermenter, xylanase activity of 12.5 IU was achieved. |
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