Production of cellulase-free xylanase by a thermotolerant Streptomyces sp. grown on agricultural waste and media optimization using mixture design and Plackett-Burman experimental design methods
Cellulase-free xylanase was produced by Streptomyces sp. Ab106 on finely ground cane bagasse at 55°C. The optimal medium composition was developed by applying the mixture design and linear mathematical program, and evaluated using the Plackett-Burman experimental design. The best composition of basa...
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2014
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th-cmuir.6653943832-5782014-08-29T08:50:24Z Production of cellulase-free xylanase by a thermotolerant Streptomyces sp. grown on agricultural waste and media optimization using mixture design and Plackett-Burman experimental design methods Techapun C. Sinsuwongwat S. Watanabe M. Sasaki K. Poosaran N. Cellulase-free xylanase was produced by Streptomyces sp. Ab106 on finely ground cane bagasse at 55°C. The optimal medium composition was developed by applying the mixture design and linear mathematical program, and evaluated using the Plackett-Burman experimental design. The best composition of basal medium was found by using the mixture design method. The highest xylanase activity, 10.6 IU, was obtained after 6 days of fermentation in shaked flask at 100 rpm, 55°C, pH 7. Both experimental designs showed that trace elements induced xylanase production. With fermentation in a 5-1 fermenter, xylanase activity of 12.5 IU was achieved. 2014-08-29T08:50:24Z 2014-08-29T08:50:24Z 2002 Article 01415492 10.1023/A:1019827321162 BILED http://www.scopus.com/inward/record.url?eid=2-s2.0-0036735527&partnerID=40&md5=60a461ba95af5b04d5f248f85186dbad http://cmuir.cmu.ac.th/handle/6653943832/578 English |
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Cellulase-free xylanase was produced by Streptomyces sp. Ab106 on finely ground cane bagasse at 55°C. The optimal medium composition was developed by applying the mixture design and linear mathematical program, and evaluated using the Plackett-Burman experimental design. The best composition of basal medium was found by using the mixture design method. The highest xylanase activity, 10.6 IU, was obtained after 6 days of fermentation in shaked flask at 100 rpm, 55°C, pH 7. Both experimental designs showed that trace elements induced xylanase production. With fermentation in a 5-1 fermenter, xylanase activity of 12.5 IU was achieved. |
| format |
Article |
| author |
Techapun C. Sinsuwongwat S. Watanabe M. Sasaki K. Poosaran N. |
| spellingShingle |
Techapun C. Sinsuwongwat S. Watanabe M. Sasaki K. Poosaran N. Production of cellulase-free xylanase by a thermotolerant Streptomyces sp. grown on agricultural waste and media optimization using mixture design and Plackett-Burman experimental design methods |
| author_facet |
Techapun C. Sinsuwongwat S. Watanabe M. Sasaki K. Poosaran N. |
| author_sort |
Techapun C. |
| title |
Production of cellulase-free xylanase by a thermotolerant Streptomyces sp. grown on agricultural waste and media optimization using mixture design and Plackett-Burman experimental design methods |
| title_short |
Production of cellulase-free xylanase by a thermotolerant Streptomyces sp. grown on agricultural waste and media optimization using mixture design and Plackett-Burman experimental design methods |
| title_full |
Production of cellulase-free xylanase by a thermotolerant Streptomyces sp. grown on agricultural waste and media optimization using mixture design and Plackett-Burman experimental design methods |
| title_fullStr |
Production of cellulase-free xylanase by a thermotolerant Streptomyces sp. grown on agricultural waste and media optimization using mixture design and Plackett-Burman experimental design methods |
| title_full_unstemmed |
Production of cellulase-free xylanase by a thermotolerant Streptomyces sp. grown on agricultural waste and media optimization using mixture design and Plackett-Burman experimental design methods |
| title_sort |
production of cellulase-free xylanase by a thermotolerant streptomyces sp. grown on agricultural waste and media optimization using mixture design and plackett-burman experimental design methods |
| publishDate |
2014 |
| url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-0036735527&partnerID=40&md5=60a461ba95af5b04d5f248f85186dbad http://cmuir.cmu.ac.th/handle/6653943832/578 |
| _version_ |
1681419509410299904 |