Systematic extraction and characterization of β-glucan from the industrial brewing yeast (Saccharomyces cerevisiae) waste material and the immunomodulatory studies on tumor necrosis factor-alpha and interleukin-6 cytokine balance of the mouse macrophage cell lines

© 2017, Faculty of Pharmaceutical Sciences, Chulalongkorn University. All rights reserved. Objectives: The aim of this investigation was to create value to the industrial waste production by systematic extraction and purification of β-glucan (BBG) from the industrial brewing yeast (Saccharomyces cer...

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Main Authors: Theeraya Krisdaphong, Toshihiko Toida, Michael Popp, Surapol Natakankitkul
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/57868
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-578682018-09-05T03:52:00Z Systematic extraction and characterization of β-glucan from the industrial brewing yeast (Saccharomyces cerevisiae) waste material and the immunomodulatory studies on tumor necrosis factor-alpha and interleukin-6 cytokine balance of the mouse macrophage cell lines Theeraya Krisdaphong Toshihiko Toida Michael Popp Surapol Natakankitkul Pharmacology, Toxicology and Pharmaceutics © 2017, Faculty of Pharmaceutical Sciences, Chulalongkorn University. All rights reserved. Objectives: The aim of this investigation was to create value to the industrial waste production by systematic extraction and purification of β-glucan (BBG) from the industrial brewing yeast (Saccharomyces cerevisiae) waste material. In addition, the immunological activity of the obtained BBG was also evaluated. Materials and Methods: The BBG from the yeast waste material of the brewing production was isolated by cell autolysis fractioning, solvent extraction, and drying. The obtained BBG was characterized by High-performance liquid chromatography, nuclear magnetic resonance and comparison with information in literature. The characterized BBG was then examined for its immunomodulatory activity on RAW 264.7 cell. Results: The optimal extraction method of BBG from the brewing waste material showed high extraction yield (13.14% w/w). In addition, the immunomodulatory study on RAW 264.7 cell lines showed that the isolated BBG could suppress the tumor necrosis factor-alpha and interleukin-6 production in the cell lines at 6 and 24 h, compared to the lipopolysaccharide control. 2018-09-05T03:52:00Z 2018-09-05T03:52:00Z 2017-01-01 Journal 19054637 01254685 2-s2.0-85030977047 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85030977047&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/57868
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Pharmacology, Toxicology and Pharmaceutics
spellingShingle Pharmacology, Toxicology and Pharmaceutics
Theeraya Krisdaphong
Toshihiko Toida
Michael Popp
Surapol Natakankitkul
Systematic extraction and characterization of β-glucan from the industrial brewing yeast (Saccharomyces cerevisiae) waste material and the immunomodulatory studies on tumor necrosis factor-alpha and interleukin-6 cytokine balance of the mouse macrophage cell lines
description © 2017, Faculty of Pharmaceutical Sciences, Chulalongkorn University. All rights reserved. Objectives: The aim of this investigation was to create value to the industrial waste production by systematic extraction and purification of β-glucan (BBG) from the industrial brewing yeast (Saccharomyces cerevisiae) waste material. In addition, the immunological activity of the obtained BBG was also evaluated. Materials and Methods: The BBG from the yeast waste material of the brewing production was isolated by cell autolysis fractioning, solvent extraction, and drying. The obtained BBG was characterized by High-performance liquid chromatography, nuclear magnetic resonance and comparison with information in literature. The characterized BBG was then examined for its immunomodulatory activity on RAW 264.7 cell. Results: The optimal extraction method of BBG from the brewing waste material showed high extraction yield (13.14% w/w). In addition, the immunomodulatory study on RAW 264.7 cell lines showed that the isolated BBG could suppress the tumor necrosis factor-alpha and interleukin-6 production in the cell lines at 6 and 24 h, compared to the lipopolysaccharide control.
format Journal
author Theeraya Krisdaphong
Toshihiko Toida
Michael Popp
Surapol Natakankitkul
author_facet Theeraya Krisdaphong
Toshihiko Toida
Michael Popp
Surapol Natakankitkul
author_sort Theeraya Krisdaphong
title Systematic extraction and characterization of β-glucan from the industrial brewing yeast (Saccharomyces cerevisiae) waste material and the immunomodulatory studies on tumor necrosis factor-alpha and interleukin-6 cytokine balance of the mouse macrophage cell lines
title_short Systematic extraction and characterization of β-glucan from the industrial brewing yeast (Saccharomyces cerevisiae) waste material and the immunomodulatory studies on tumor necrosis factor-alpha and interleukin-6 cytokine balance of the mouse macrophage cell lines
title_full Systematic extraction and characterization of β-glucan from the industrial brewing yeast (Saccharomyces cerevisiae) waste material and the immunomodulatory studies on tumor necrosis factor-alpha and interleukin-6 cytokine balance of the mouse macrophage cell lines
title_fullStr Systematic extraction and characterization of β-glucan from the industrial brewing yeast (Saccharomyces cerevisiae) waste material and the immunomodulatory studies on tumor necrosis factor-alpha and interleukin-6 cytokine balance of the mouse macrophage cell lines
title_full_unstemmed Systematic extraction and characterization of β-glucan from the industrial brewing yeast (Saccharomyces cerevisiae) waste material and the immunomodulatory studies on tumor necrosis factor-alpha and interleukin-6 cytokine balance of the mouse macrophage cell lines
title_sort systematic extraction and characterization of β-glucan from the industrial brewing yeast (saccharomyces cerevisiae) waste material and the immunomodulatory studies on tumor necrosis factor-alpha and interleukin-6 cytokine balance of the mouse macrophage cell lines
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85030977047&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/57868
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