Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
2014
|
| Online Access: | http://www.scopus.com/inward/record.url?eid=2-s2.0-70349789724&partnerID=40&md5=2de8ebf2670d6e7ef619db7268ba24ae http://www.ncbi.nlm.nih.gov/pubmed/19842374 http://cmuir.cmu.ac.th/handle/6653943832/5822 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Chiang Mai University |
| Language: | English |
| Summary: | Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A 256 bp HAT-RAPD marker generated from OPP-11 primer specific for H. taichui was cloned and sequenced. From the sequence data, specific primers were designed that generated a 256 bp amplicon. The minimum DNA template needed for PCR detection was 10 fg. The successful development of the H. taichui specific DNA-based detection will be beneficial in management and epidemiological control programs. |
|---|