Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui

Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A...

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Main Authors:	Wongsawad P., Wongsawad C.
Format:	Article
Language:	English
Published:	2014
Online Access:	http://www.scopus.com/inward/record.url?eid=2-s2.0-70349789724&partnerID=40&md5=2de8ebf2670d6e7ef619db7268ba24ae http://www.ncbi.nlm.nih.gov/pubmed/19842374 http://cmuir.cmu.ac.th/handle/6653943832/5822
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Institution:	Chiang Mai University
Language:	English

id	th-cmuir.6653943832-5822
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spelling	th-cmuir.6653943832-58222014-08-30T03:23:30Z Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui Wongsawad P. Wongsawad C. Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A 256 bp HAT-RAPD marker generated from OPP-11 primer specific for H. taichui was cloned and sequenced. From the sequence data, specific primers were designed that generated a 256 bp amplicon. The minimum DNA template needed for PCR detection was 10 fg. The successful development of the H. taichui specific DNA-based detection will be beneficial in management and epidemiological control programs. 2014-08-30T03:23:30Z 2014-08-30T03:23:30Z 2009 Article 01251562 19842374 http://www.scopus.com/inward/record.url?eid=2-s2.0-70349789724&partnerID=40&md5=2de8ebf2670d6e7ef619db7268ba24ae http://www.ncbi.nlm.nih.gov/pubmed/19842374 http://cmuir.cmu.ac.th/handle/6653943832/5822 English
institution	Chiang Mai University
building	Chiang Mai University Library
country	Thailand
collection	CMU Intellectual Repository
language	English
description	Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A 256 bp HAT-RAPD marker generated from OPP-11 primer specific for H. taichui was cloned and sequenced. From the sequence data, specific primers were designed that generated a 256 bp amplicon. The minimum DNA template needed for PCR detection was 10 fg. The successful development of the H. taichui specific DNA-based detection will be beneficial in management and epidemiological control programs.
format	Article
author	Wongsawad P. Wongsawad C.
spellingShingle	Wongsawad P. Wongsawad C. Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
author_facet	Wongsawad P. Wongsawad C.
author_sort	Wongsawad P.
title	Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
title_short	Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
title_full	Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
title_fullStr	Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
title_full_unstemmed	Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
title_sort	development of pcr-based diagnosis of minute intestinal fluke, haplorchis taichui
publishDate	2014
url	http://www.scopus.com/inward/record.url?eid=2-s2.0-70349789724&partnerID=40&md5=2de8ebf2670d6e7ef619db7268ba24ae http://www.ncbi.nlm.nih.gov/pubmed/19842374 http://cmuir.cmu.ac.th/handle/6653943832/5822
_version_	1681420498167136256

Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui

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