A modified sandwich ELISA for accurate measurement of HbF in α-thalassemia carriers containing Hb Bart’s and Hb Portland 1
© 2018, © 2018 Taylor & Francis. Hemoglobin F (HbF) in blood lysate can be accurately measured by various methods, including immunoassay. In this study, we have produced polyclonal antibody (pAb) against HbF and established a modified sandwich-type ELISA for HbF quantification in blood lysates...
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th-cmuir.6653943832-582482018-09-05T04:35:05Z A modified sandwich ELISA for accurate measurement of HbF in α-thalassemia carriers containing Hb Bart’s and Hb Portland 1 Sasiwan Kerdpoo Witida Laopajon Watchara Kasinrerk Supansa Pata Thanusak Tatu Biochemistry, Genetics and Molecular Biology Health Professions Immunology and Microbiology Medicine © 2018, © 2018 Taylor & Francis. Hemoglobin F (HbF) in blood lysate can be accurately measured by various methods, including immunoassay. In this study, we have produced polyclonal antibody (pAb) against HbF and established a modified sandwich-type ELISA for HbF quantification in blood lysates. The modified sandwich ELISA utilized anti-γ-globin monoclonal antibody clones Thal N/B as the capture antibody (Ab) coated on solid-phase, fluorescein isothiocyanate (FITC)-labeled pAb as the detecting Ab, and HPR-labeled anti-FITC Ab as the signal-generating Ab. By using an optimized blood lysate dilution, the HbF could be measured with no interference from hemoglobin Bart’s (Hb Bart’s) and hemoglobin Portland (Hb Portland 1) presented in α-thalassemia carriers. HbF levels measured by the modified sandwich ELISA were comparable to those quantified by the standard cation-exchange high performance liquid chromatography. We suggested that this modified sandwich ELISA was able to accurately measure HbF levels even in α-thalassemia carriers containing Hb Bart’s and Hb Portland 1 and be an alternative method for HbF measurement. 2018-09-05T04:21:40Z 2018-09-05T04:21:40Z 2018-05-04 Journal 15324230 15321819 2-s2.0-85049616434 10.1080/15321819.2018.1488726 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85049616434&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/58248 |
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Biochemistry, Genetics and Molecular Biology Health Professions Immunology and Microbiology Medicine Sasiwan Kerdpoo Witida Laopajon Watchara Kasinrerk Supansa Pata Thanusak Tatu A modified sandwich ELISA for accurate measurement of HbF in α-thalassemia carriers containing Hb Bart’s and Hb Portland 1 |
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© 2018, © 2018 Taylor & Francis. Hemoglobin F (HbF) in blood lysate can be accurately measured by various methods, including immunoassay. In this study, we have produced polyclonal antibody (pAb) against HbF and established a modified sandwich-type ELISA for HbF quantification in blood lysates. The modified sandwich ELISA utilized anti-γ-globin monoclonal antibody clones Thal N/B as the capture antibody (Ab) coated on solid-phase, fluorescein isothiocyanate (FITC)-labeled pAb as the detecting Ab, and HPR-labeled anti-FITC Ab as the signal-generating Ab. By using an optimized blood lysate dilution, the HbF could be measured with no interference from hemoglobin Bart’s (Hb Bart’s) and hemoglobin Portland (Hb Portland 1) presented in α-thalassemia carriers. HbF levels measured by the modified sandwich ELISA were comparable to those quantified by the standard cation-exchange high performance liquid chromatography. We suggested that this modified sandwich ELISA was able to accurately measure HbF levels even in α-thalassemia carriers containing Hb Bart’s and Hb Portland 1 and be an alternative method for HbF measurement. |
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Sasiwan Kerdpoo Witida Laopajon Watchara Kasinrerk Supansa Pata Thanusak Tatu |
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Sasiwan Kerdpoo Witida Laopajon Watchara Kasinrerk Supansa Pata Thanusak Tatu |
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Sasiwan Kerdpoo |
title |
A modified sandwich ELISA for accurate measurement of HbF in α-thalassemia carriers containing Hb Bart’s and Hb Portland 1 |
title_short |
A modified sandwich ELISA for accurate measurement of HbF in α-thalassemia carriers containing Hb Bart’s and Hb Portland 1 |
title_full |
A modified sandwich ELISA for accurate measurement of HbF in α-thalassemia carriers containing Hb Bart’s and Hb Portland 1 |
title_fullStr |
A modified sandwich ELISA for accurate measurement of HbF in α-thalassemia carriers containing Hb Bart’s and Hb Portland 1 |
title_full_unstemmed |
A modified sandwich ELISA for accurate measurement of HbF in α-thalassemia carriers containing Hb Bart’s and Hb Portland 1 |
title_sort |
modified sandwich elisa for accurate measurement of hbf in α-thalassemia carriers containing hb bart’s and hb portland 1 |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85049616434&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/58248 |
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