Development of immunochromatographic assay for the on-site detection of salbutamol

Salbutamol, one of the β-agonists, is misused as a growth promoter in meat producing animals. In-house synthesized colloidal gold was conjugated with the polyclonal anti-salbutamol antibodies. A rapid immunochromatographic assay was developed in a competitive format. The salbutamol-BSA conjugate and...

Full description

Saved in:
Bibliographic Details
Main Authors: Yaowapa Khamta, Mookda Pattarawarapan, Sawitree Nangola, Chatchai Tayapiwatana
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70449730062&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59340
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
Description
Summary:Salbutamol, one of the β-agonists, is misused as a growth promoter in meat producing animals. In-house synthesized colloidal gold was conjugated with the polyclonal anti-salbutamol antibodies. A rapid immunochromatographic assay was developed in a competitive format. The salbutamol-BSA conjugate and goat anti-rabbit IgG were immobilized on a nitrocellulose membrane as test and control lines, respectively. The color intensity of a purple test line was inversely proportional to the amount of salbutamol presenting in the samples. The sensitivity was estimated to be about 80ng/mL of salbutamol in PBS. The method can be useful as an on-site screening procedure for detection of salbutamol.