Development of immunochromatographic assay for the on-site detection of salbutamol

Salbutamol, one of the β-agonists, is misused as a growth promoter in meat producing animals. In-house synthesized colloidal gold was conjugated with the polyclonal anti-salbutamol antibodies. A rapid immunochromatographic assay was developed in a competitive format. The salbutamol-BSA conjugate and...

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Main Authors:	Yaowapa Khamta, Mookda Pattarawarapan, Sawitree Nangola, Chatchai Tayapiwatana
Format:	Journal
Published:	2018
Subjects:	Biochemistry, Genetics and Molecular Biology Health Professions Immunology and Microbiology Medicine
Online Access:	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70449730062&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59340
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Institution:	Chiang Mai University

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spelling	th-cmuir.6653943832-593402018-09-10T03:21:32Z Development of immunochromatographic assay for the on-site detection of salbutamol Yaowapa Khamta Mookda Pattarawarapan Sawitree Nangola Chatchai Tayapiwatana Biochemistry, Genetics and Molecular Biology Health Professions Immunology and Microbiology Medicine Salbutamol, one of the β-agonists, is misused as a growth promoter in meat producing animals. In-house synthesized colloidal gold was conjugated with the polyclonal anti-salbutamol antibodies. A rapid immunochromatographic assay was developed in a competitive format. The salbutamol-BSA conjugate and goat anti-rabbit IgG were immobilized on a nitrocellulose membrane as test and control lines, respectively. The color intensity of a purple test line was inversely proportional to the amount of salbutamol presenting in the samples. The sensitivity was estimated to be about 80ng/mL of salbutamol in PBS. The method can be useful as an on-site screening procedure for detection of salbutamol. 2018-09-10T03:14:02Z 2018-09-10T03:14:02Z 2009-10-01 Journal 15324230 15321819 2-s2.0-70449730062 10.1080/15321810903188284 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70449730062&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59340
institution	Chiang Mai University
building	Chiang Mai University Library
country	Thailand
collection	CMU Intellectual Repository
topic	Biochemistry, Genetics and Molecular Biology Health Professions Immunology and Microbiology Medicine
spellingShingle	Biochemistry, Genetics and Molecular Biology Health Professions Immunology and Microbiology Medicine Yaowapa Khamta Mookda Pattarawarapan Sawitree Nangola Chatchai Tayapiwatana Development of immunochromatographic assay for the on-site detection of salbutamol
description	Salbutamol, one of the β-agonists, is misused as a growth promoter in meat producing animals. In-house synthesized colloidal gold was conjugated with the polyclonal anti-salbutamol antibodies. A rapid immunochromatographic assay was developed in a competitive format. The salbutamol-BSA conjugate and goat anti-rabbit IgG were immobilized on a nitrocellulose membrane as test and control lines, respectively. The color intensity of a purple test line was inversely proportional to the amount of salbutamol presenting in the samples. The sensitivity was estimated to be about 80ng/mL of salbutamol in PBS. The method can be useful as an on-site screening procedure for detection of salbutamol.
format	Journal
author	Yaowapa Khamta Mookda Pattarawarapan Sawitree Nangola Chatchai Tayapiwatana
author_facet	Yaowapa Khamta Mookda Pattarawarapan Sawitree Nangola Chatchai Tayapiwatana
author_sort	Yaowapa Khamta
title	Development of immunochromatographic assay for the on-site detection of salbutamol
title_short	Development of immunochromatographic assay for the on-site detection of salbutamol
title_full	Development of immunochromatographic assay for the on-site detection of salbutamol
title_fullStr	Development of immunochromatographic assay for the on-site detection of salbutamol
title_full_unstemmed	Development of immunochromatographic assay for the on-site detection of salbutamol
title_sort	development of immunochromatographic assay for the on-site detection of salbutamol
publishDate	2018
url	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70449730062&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59340
_version_	1681425233073930240

Development of immunochromatographic assay for the on-site detection of salbutamol

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