Isolation of thermotolerant acetic acid bacteria from fruits for vinegar production

Sixty thermotolerant acetic acid bacteria were isolated from 13 kinds of fruit using sterile distilled water supplemented with 4% ethanol (v/v) as an enrichment medium. Successful isolations were obtained from apple, Jamaican cherry, longan, mango, pineapple and rambutan. Morphological and biochemic...

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Main Authors: Amornrut Moryadee, Wasu Pathom-Aree
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/60112
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spelling th-cmuir.6653943832-601122018-09-10T03:47:16Z Isolation of thermotolerant acetic acid bacteria from fruits for vinegar production Amornrut Moryadee Wasu Pathom-Aree Agricultural and Biological Sciences Immunology and Microbiology Medicine Sixty thermotolerant acetic acid bacteria were isolated from 13 kinds of fruit using sterile distilled water supplemented with 4% ethanol (v/v) as an enrichment medium. Successful isolations were obtained from apple, Jamaican cherry, longan, mango, pineapple and rambutan. Morphological and biochemical examinations revealed that 43 isolates were members of the genus Acetobacter whereas the remaining 13 isolates were members of the genus Gluconobacter. Preliminary screening showed that isolates No. 13, 34, 36 and 37 gave the widest zone of acidity on overoxidation medium. These isolates were identified as A. aceti and selected for acetic acid production at 30 and 37°C by shaking culture for 14 days in ethanol-yeast extract medium. It was found that A. aceti isolate No. 37 from rambutan gave the highest acetic acid yield of 13.53 and 8.97 g L-1at 30 and 37°C, respectively after 7 days of fermentation. © 2008 Academic Journals Inc. 2018-09-10T03:38:13Z 2018-09-10T03:38:13Z 2008-01-01 Journal 18164935 2-s2.0-40949154806 10.3923/jm.2008.209.212 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=40949154806&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60112
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Immunology and Microbiology
Medicine
spellingShingle Agricultural and Biological Sciences
Immunology and Microbiology
Medicine
Amornrut Moryadee
Wasu Pathom-Aree
Isolation of thermotolerant acetic acid bacteria from fruits for vinegar production
description Sixty thermotolerant acetic acid bacteria were isolated from 13 kinds of fruit using sterile distilled water supplemented with 4% ethanol (v/v) as an enrichment medium. Successful isolations were obtained from apple, Jamaican cherry, longan, mango, pineapple and rambutan. Morphological and biochemical examinations revealed that 43 isolates were members of the genus Acetobacter whereas the remaining 13 isolates were members of the genus Gluconobacter. Preliminary screening showed that isolates No. 13, 34, 36 and 37 gave the widest zone of acidity on overoxidation medium. These isolates were identified as A. aceti and selected for acetic acid production at 30 and 37°C by shaking culture for 14 days in ethanol-yeast extract medium. It was found that A. aceti isolate No. 37 from rambutan gave the highest acetic acid yield of 13.53 and 8.97 g L-1at 30 and 37°C, respectively after 7 days of fermentation. © 2008 Academic Journals Inc.
format Journal
author Amornrut Moryadee
Wasu Pathom-Aree
author_facet Amornrut Moryadee
Wasu Pathom-Aree
author_sort Amornrut Moryadee
title Isolation of thermotolerant acetic acid bacteria from fruits for vinegar production
title_short Isolation of thermotolerant acetic acid bacteria from fruits for vinegar production
title_full Isolation of thermotolerant acetic acid bacteria from fruits for vinegar production
title_fullStr Isolation of thermotolerant acetic acid bacteria from fruits for vinegar production
title_full_unstemmed Isolation of thermotolerant acetic acid bacteria from fruits for vinegar production
title_sort isolation of thermotolerant acetic acid bacteria from fruits for vinegar production
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=40949154806&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60112
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