Interobserver reproducibility with LiquiPrep™ liquid-based cervical cytology screening in a developing country

Objective: A modified liquid-based techniques known as the "LiquiPrep™ (LP) system" requires neither expensive equipment nor complicated specimen preparation. The aim of this study was to assess the applicability of the LP for use in a developing country. Methods: Cervical cytology specime...

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Main Authors: Jongkolnee Settakorn, Samreung Rangdaeng, Natchanun Preechapornkul, Sudaporn Nateewatana, Kampol Pongsiralai, Jatupol Srisomboon, Paul S. Thorner
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/60194
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-601942018-09-10T03:47:13Z Interobserver reproducibility with LiquiPrep™ liquid-based cervical cytology screening in a developing country Jongkolnee Settakorn Samreung Rangdaeng Natchanun Preechapornkul Sudaporn Nateewatana Kampol Pongsiralai Jatupol Srisomboon Paul S. Thorner Biochemistry, Genetics and Molecular Biology Medicine Objective: A modified liquid-based techniques known as the "LiquiPrep™ (LP) system" requires neither expensive equipment nor complicated specimen preparation. The aim of this study was to assess the applicability of the LP for use in a developing country. Methods: Cervical cytology specimens were collected from 777 women, using the Cervex-Brush™. The brush was first smeared on a glass side for conventional Papanicolaou (CP) stain, and then immersed in preservation fluid for LP preparation. Cytologic interpretations were classified into four categories: 1) no atypical cells, 2) atypical squamous epithelial cells (ASC), 3) definite epithelial cell abnormality, and 4) unsatisfactory specimen. Interobserver variability was tested using weighted kappa statistics. Results: An LP specimen cost $9 per case compared to $3 per case for a conventional Pap smear. The time to learn the technique was only a few days. Forty six (5.92%) specimens by LP were unsatisfactory. The overall agreement between cytopathologists was 96.7% (weighted κ=0.62), with 95.6% (weighted κ=0.44) for the cases enrolled earlier, increasing to 97.9% (weighted κ=0.78) for the cases enrolled later. Conclusions: In summary, after a short learning curve, interobserver reproducibility of LP smear was near perfect. This feature of the LP, together with the relatively low cost and simple protocol, makes it quite suitable for cervical cytology screening in developing countries. Moreover, with this technique, some of each sample can be reserved for additional studies such as HPV detection and subtyping. 2018-09-10T03:39:11Z 2018-09-10T03:39:11Z 2008-01-01 Journal 2476762X 15137368 2-s2.0-58349106332 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=58349106332&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60194
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Jongkolnee Settakorn
Samreung Rangdaeng
Natchanun Preechapornkul
Sudaporn Nateewatana
Kampol Pongsiralai
Jatupol Srisomboon
Paul S. Thorner
Interobserver reproducibility with LiquiPrep™ liquid-based cervical cytology screening in a developing country
description Objective: A modified liquid-based techniques known as the "LiquiPrep™ (LP) system" requires neither expensive equipment nor complicated specimen preparation. The aim of this study was to assess the applicability of the LP for use in a developing country. Methods: Cervical cytology specimens were collected from 777 women, using the Cervex-Brush™. The brush was first smeared on a glass side for conventional Papanicolaou (CP) stain, and then immersed in preservation fluid for LP preparation. Cytologic interpretations were classified into four categories: 1) no atypical cells, 2) atypical squamous epithelial cells (ASC), 3) definite epithelial cell abnormality, and 4) unsatisfactory specimen. Interobserver variability was tested using weighted kappa statistics. Results: An LP specimen cost $9 per case compared to $3 per case for a conventional Pap smear. The time to learn the technique was only a few days. Forty six (5.92%) specimens by LP were unsatisfactory. The overall agreement between cytopathologists was 96.7% (weighted κ=0.62), with 95.6% (weighted κ=0.44) for the cases enrolled earlier, increasing to 97.9% (weighted κ=0.78) for the cases enrolled later. Conclusions: In summary, after a short learning curve, interobserver reproducibility of LP smear was near perfect. This feature of the LP, together with the relatively low cost and simple protocol, makes it quite suitable for cervical cytology screening in developing countries. Moreover, with this technique, some of each sample can be reserved for additional studies such as HPV detection and subtyping.
format Journal
author Jongkolnee Settakorn
Samreung Rangdaeng
Natchanun Preechapornkul
Sudaporn Nateewatana
Kampol Pongsiralai
Jatupol Srisomboon
Paul S. Thorner
author_facet Jongkolnee Settakorn
Samreung Rangdaeng
Natchanun Preechapornkul
Sudaporn Nateewatana
Kampol Pongsiralai
Jatupol Srisomboon
Paul S. Thorner
author_sort Jongkolnee Settakorn
title Interobserver reproducibility with LiquiPrep™ liquid-based cervical cytology screening in a developing country
title_short Interobserver reproducibility with LiquiPrep™ liquid-based cervical cytology screening in a developing country
title_full Interobserver reproducibility with LiquiPrep™ liquid-based cervical cytology screening in a developing country
title_fullStr Interobserver reproducibility with LiquiPrep™ liquid-based cervical cytology screening in a developing country
title_full_unstemmed Interobserver reproducibility with LiquiPrep™ liquid-based cervical cytology screening in a developing country
title_sort interobserver reproducibility with liquiprep™ liquid-based cervical cytology screening in a developing country
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=58349106332&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60194
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