Purification and characterization of thermostable α-galactosidase from Ganoderma lucidum

• Main Authors: Sripuan T., Aoki K., Yamamoto K., Tongkao D., Kumagai H.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-2442419823&partnerID=40&md5=2d81fd7bbae1df8033661e67f49e9282; http://cmuir.cmu.ac.th/handle/6653943832/6040
• Institution: Chiang Mai University

α-Galactosidase was purified from a fresh fruiting body of Ganoderma lucidum by precipitation with ammonium sulfate and column chromatographies with DEAE-Sephadex and Con A-Sepharose. The purified enzyme was homogeneous on polyacrylamide gel electrophoresis. Its N-terminal amino acid sequence was si...