Hemoglobin E detection using PCR with confronting two-pair primers
Objective: To develop and apply the polymerase chain reaction with confronting two-pair primers (PCR-CTPP) for detection and identification of hemoglobin E (Hb E). Material and Method: Fifty unrelated northern Thais were included in the present study. DNA was extracted from peripheral blood mononucl...
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| Main Authors: | , , , |
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| Format: | Journal |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=57149086322&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60573 |
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| Institution: | Chiang Mai University |
| Summary: | Objective: To develop and apply the polymerase chain reaction with confronting two-pair primers (PCR-CTPP) for detection and identification of hemoglobin E (Hb E). Material and Method: Fifty unrelated northern Thais were included in the present study. DNA was extracted from peripheral blood mononuclear cells and targeted to amplify by PCR-CTPP. The amplified product was analyzed and compared with the reference hemoglobin electrophoresis and polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) analysis. Results: The results validated a completely concordant among these three methods consisting of 74%, 24%, and 2% identified as normal, heterozygous, and homozygous Hb E type, respectively. Conclusion: Successful Hb E genotyping by PCR-CTPP was introduced. It allows for confirming and simultaneously detection with other thalassemia mutations. |
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