Hemoglobin E detection using PCR with confronting two-pair primers

Hemoglobin E detection using PCR with confronting two-pair primers

Objective: To develop and apply the polymerase chain reaction with confronting two-pair primers (PCR-CTPP) for detection and identification of hemoglobin E (Hb E). Material and Method: Fifty unrelated northern Thais were included in the present study. DNA was extracted from peripheral blood mononucl...

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Main Authors: Sorasak Intorasoot, Rachanu Thongpung, Khajornsak Tragoolpua, Mongkol Chottayaporn
Format: Journal
Published: 2018
Subjects:
Medicine
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=57149086322&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60573
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-605732018-09-10T03:45:35Z Hemoglobin E detection using PCR with confronting two-pair primers Sorasak Intorasoot Rachanu Thongpung Khajornsak Tragoolpua Mongkol Chottayaporn Medicine Objective: To develop and apply the polymerase chain reaction with confronting two-pair primers (PCR-CTPP) for detection and identification of hemoglobin E (Hb E). Material and Method: Fifty unrelated northern Thais were included in the present study. DNA was extracted from peripheral blood mononuclear cells and targeted to amplify by PCR-CTPP. The amplified product was analyzed and compared with the reference hemoglobin electrophoresis and polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) analysis. Results: The results validated a completely concordant among these three methods consisting of 74%, 24%, and 2% identified as normal, heterozygous, and homozygous Hb E type, respectively. Conclusion: Successful Hb E genotyping by PCR-CTPP was introduced. It allows for confirming and simultaneously detection with other thalassemia mutations. 2018-09-10T03:45:35Z 2018-09-10T03:45:35Z 2008-11-01 Journal 01252208 01252208 2-s2.0-57149086322 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=57149086322&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60573
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Sorasak Intorasoot
Rachanu Thongpung
Khajornsak Tragoolpua
Mongkol Chottayaporn
Hemoglobin E detection using PCR with confronting two-pair primers
description Objective: To develop and apply the polymerase chain reaction with confronting two-pair primers (PCR-CTPP) for detection and identification of hemoglobin E (Hb E). Material and Method: Fifty unrelated northern Thais were included in the present study. DNA was extracted from peripheral blood mononuclear cells and targeted to amplify by PCR-CTPP. The amplified product was analyzed and compared with the reference hemoglobin electrophoresis and polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) analysis. Results: The results validated a completely concordant among these three methods consisting of 74%, 24%, and 2% identified as normal, heterozygous, and homozygous Hb E type, respectively. Conclusion: Successful Hb E genotyping by PCR-CTPP was introduced. It allows for confirming and simultaneously detection with other thalassemia mutations.
format Journal
author Sorasak Intorasoot
Rachanu Thongpung
Khajornsak Tragoolpua
Mongkol Chottayaporn
author_facet Sorasak Intorasoot
Rachanu Thongpung
Khajornsak Tragoolpua
Mongkol Chottayaporn
author_sort Sorasak Intorasoot
title Hemoglobin E detection using PCR with confronting two-pair primers
title_short Hemoglobin E detection using PCR with confronting two-pair primers
title_full Hemoglobin E detection using PCR with confronting two-pair primers
title_fullStr Hemoglobin E detection using PCR with confronting two-pair primers
title_full_unstemmed Hemoglobin E detection using PCR with confronting two-pair primers
title_sort hemoglobin e detection using pcr with confronting two-pair primers
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=57149086322&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60573
_version_ 1681425460787937280

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