Solid-to-solid peptide synthesis by glycyl endopeptidase

• Main Authors: Phanuphong Chaiwut, Pawinee Kanasawud, Peter J. Halling
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33847354740&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/60913
• Institution: Chiang Mai University

Glycyl endopeptidase catalysed solid-to-solid synthesis can be carried out efficiently for the model peptides Z-Gly-Phe-NH2, Z-Gly-Leu-NH2, Z-Gly-Tyr-NH2and Z-Gly-Tyr-OEt. A small excess of acyl donor Z-Gly improved both the initial rate and final yield, whereas an excess of nucleophile Phe-NH2preve...