The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand

Hyperthermophilic community diversity was assessed in hot-spring streamers along gradients of temperature, pH and sulphide in northern Thailand. A hierarchical sampling design was employed to obtain biomass for culture-independent estimates of 16S rRNA gene-defined prokaryotic diversity. All springs...

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Main Authors: Diane Purcell, Udomluk Sompong, Lau Chui Yim, Timothy G. Barraclough, Yuwadee Peerapornpisal, Stephen B. Pointing
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/61102
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-611022018-09-10T04:05:16Z The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand Diane Purcell Udomluk Sompong Lau Chui Yim Timothy G. Barraclough Yuwadee Peerapornpisal Stephen B. Pointing Environmental Science Immunology and Microbiology Hyperthermophilic community diversity was assessed in hot-spring streamers along gradients of temperature, pH and sulphide in northern Thailand. A hierarchical sampling design was employed to obtain biomass for culture-independent estimates of 16S rRNA gene-defined prokaryotic diversity. All springs supported several archaeal and bacterial phylotypes, including novel phylotypes that expand the known phylogenetic diversity of terrestrial hyperthermophiles. Diversity appeared significantly greater than that observed for several other geographic locations. Phylotypes belonging to the Aquificales were ubiquitous, further supporting the hypothesis that these chemolithoautotrophs are key members of all hyperthermophilic communities. The chemoorganotrophic genus Thermus was also represented by phylotypes in all springs. Other bacterial taxa represented by environmental sequences included Bacillus, Thermotoga and various unidentified Alphaproteobacteria and Betaproteobacteria. Archaeal phylotypes included the Crenarchaea Desulfurococcus, Pyrobaculum, plus several unidentified hyperthermophilic lineages. A Methanothermococcus-like Euryarchaeon was also identified, with this genus not previously known from streamer communities. A multivariate approach to the analysis of biotic and abiotic data revealed that diversity patterns were best explained by a combination of temperature and sulphide rather than by any other abiotic variable either individually or in combination. © 2007 Federation of European Microbiological Societies. 2018-09-10T04:04:24Z 2018-09-10T04:04:24Z 2007-06-01 Journal 15746941 01686496 2-s2.0-34249095755 10.1111/j.1574-6941.2007.00302.x https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34249095755&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61102
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Environmental Science
Immunology and Microbiology
spellingShingle Environmental Science
Immunology and Microbiology
Diane Purcell
Udomluk Sompong
Lau Chui Yim
Timothy G. Barraclough
Yuwadee Peerapornpisal
Stephen B. Pointing
The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand
description Hyperthermophilic community diversity was assessed in hot-spring streamers along gradients of temperature, pH and sulphide in northern Thailand. A hierarchical sampling design was employed to obtain biomass for culture-independent estimates of 16S rRNA gene-defined prokaryotic diversity. All springs supported several archaeal and bacterial phylotypes, including novel phylotypes that expand the known phylogenetic diversity of terrestrial hyperthermophiles. Diversity appeared significantly greater than that observed for several other geographic locations. Phylotypes belonging to the Aquificales were ubiquitous, further supporting the hypothesis that these chemolithoautotrophs are key members of all hyperthermophilic communities. The chemoorganotrophic genus Thermus was also represented by phylotypes in all springs. Other bacterial taxa represented by environmental sequences included Bacillus, Thermotoga and various unidentified Alphaproteobacteria and Betaproteobacteria. Archaeal phylotypes included the Crenarchaea Desulfurococcus, Pyrobaculum, plus several unidentified hyperthermophilic lineages. A Methanothermococcus-like Euryarchaeon was also identified, with this genus not previously known from streamer communities. A multivariate approach to the analysis of biotic and abiotic data revealed that diversity patterns were best explained by a combination of temperature and sulphide rather than by any other abiotic variable either individually or in combination. © 2007 Federation of European Microbiological Societies.
format Journal
author Diane Purcell
Udomluk Sompong
Lau Chui Yim
Timothy G. Barraclough
Yuwadee Peerapornpisal
Stephen B. Pointing
author_facet Diane Purcell
Udomluk Sompong
Lau Chui Yim
Timothy G. Barraclough
Yuwadee Peerapornpisal
Stephen B. Pointing
author_sort Diane Purcell
title The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand
title_short The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand
title_full The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand
title_fullStr The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand
title_full_unstemmed The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand
title_sort effects of temperature, ph and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern thailand
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34249095755&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61102
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