Cloning of D-glucose dehydrogenase with a narrow substrate specificity from Bacillus thuringiensis M15

We have cloned an ORF of Bacillus thuringiensis M15, which encodes a protein sharing high similarity with D-glucose dehydrogenase. A high-expression plasmid (pBtGDH) for the ORF was constructed. Escherichia coli JM 109 transformed with pBtGDH exhibited D-glucose dehydrogenase activity, and the enzym...

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Main Authors: Nitaya Boontim, Kazuaki Yoshimune, Saisamorn Lumyong, Mitsuaki Moriguchi
Format: Journal
Published: 2018
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Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33750194390&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61727
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Institution: Chiang Mai University

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