Characterization of salt-tolerant glutaminase from Stenotrophomonas maltophilia NYW-81 and its application in Japanese soy sauce fermentation

• Main Authors: Mamoru Wakayama, Tomohiro Yamagata, Aki Kamemura, Nitaya Bootim, Shigekazu Yano, Takashi Tachiki, Kazuaki Yoshimune, Mitsuaki Moriguchi
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=28344445175&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/62088
• Institution: Chiang Mai University

Glutaminase from Stenotrophomonas maltophilia NYW-81 was purified to homogeneity with a final specific activity of 325 U/mg. The molecular mass of the native enzyme was estimated to be 41 kDa by gel filtration. A subunit molecular mass of 36 kDa was measured with SDS-PAGE, thus indicating that the n...