Expression of fluorescent tagged recombinant erythroferrone protein

© 2018 by the Asian Pacific Journal of Tropical Biomedicine. Objective: To produce fluorescent tagged recombinant erythroferrone protein (ERFE-eGFP) for laboratory investigations. Methods: Erythroferrone (ERFE) gene was fused to green fluorescent protein (eGFP) gene and cloned in a pSecTag2Hygro pla...

Full description

Saved in:
Bibliographic Details
Main Authors: Min Min Than, Jetsada Ruangsuriya, Chairat Uthaipibull, Somdet Srichairatanakool
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85055036712&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/62583
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-62583
record_format dspace
spelling th-cmuir.6653943832-625832018-11-29T07:33:08Z Expression of fluorescent tagged recombinant erythroferrone protein Min Min Than Jetsada Ruangsuriya Chairat Uthaipibull Somdet Srichairatanakool Biochemistry, Genetics and Molecular Biology © 2018 by the Asian Pacific Journal of Tropical Biomedicine. Objective: To produce fluorescent tagged recombinant erythroferrone protein (ERFE-eGFP) for laboratory investigations. Methods: Erythroferrone (ERFE) gene was fused to green fluorescent protein (eGFP) gene and cloned in a pSecTag2Hygro plasmid. The constructed plasmid was amplified in Escherichia coli DH5 α and the eGFP-fused ERFE (ERFE-eGFP) protein was expressed in human embryonic kidney (HEK293T) cell line. Results: The plasmid constructed from colony C6 contained ERFE-eGFP with the correct restriction sizes of 4.2 kb and expressed secretory ERFE-eGFP fusion protein (approximately size of 75 kDa) in HEK293T cell line. Conclusions: ERFE-eGFP recombinant protein is successfully expressed as a secretory functional protein and could be sensitively detected using fluorometry. This fusion protein might benefit future applications for localization of cellular ERFE receptors and competitive immunoassay of ERFE concentration. 2018-11-29T07:33:08Z 2018-11-29T07:33:08Z 2018-07-01 Journal 22211691 2-s2.0-85055036712 10.4103/2221-1691.237079 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85055036712&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62583
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
spellingShingle Biochemistry, Genetics and Molecular Biology
Min Min Than
Jetsada Ruangsuriya
Chairat Uthaipibull
Somdet Srichairatanakool
Expression of fluorescent tagged recombinant erythroferrone protein
description © 2018 by the Asian Pacific Journal of Tropical Biomedicine. Objective: To produce fluorescent tagged recombinant erythroferrone protein (ERFE-eGFP) for laboratory investigations. Methods: Erythroferrone (ERFE) gene was fused to green fluorescent protein (eGFP) gene and cloned in a pSecTag2Hygro plasmid. The constructed plasmid was amplified in Escherichia coli DH5 α and the eGFP-fused ERFE (ERFE-eGFP) protein was expressed in human embryonic kidney (HEK293T) cell line. Results: The plasmid constructed from colony C6 contained ERFE-eGFP with the correct restriction sizes of 4.2 kb and expressed secretory ERFE-eGFP fusion protein (approximately size of 75 kDa) in HEK293T cell line. Conclusions: ERFE-eGFP recombinant protein is successfully expressed as a secretory functional protein and could be sensitively detected using fluorometry. This fusion protein might benefit future applications for localization of cellular ERFE receptors and competitive immunoassay of ERFE concentration.
format Journal
author Min Min Than
Jetsada Ruangsuriya
Chairat Uthaipibull
Somdet Srichairatanakool
author_facet Min Min Than
Jetsada Ruangsuriya
Chairat Uthaipibull
Somdet Srichairatanakool
author_sort Min Min Than
title Expression of fluorescent tagged recombinant erythroferrone protein
title_short Expression of fluorescent tagged recombinant erythroferrone protein
title_full Expression of fluorescent tagged recombinant erythroferrone protein
title_fullStr Expression of fluorescent tagged recombinant erythroferrone protein
title_full_unstemmed Expression of fluorescent tagged recombinant erythroferrone protein
title_sort expression of fluorescent tagged recombinant erythroferrone protein
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85055036712&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/62583
_version_ 1681425834230939648