Anti-inflammatory and antioxidant activities of the extracts from leaves and stems of Polygonum odoratum Lour

© 2019 Bentham Science Publishers. Background: Polygonum odoratum is an indigenous vegetable that has been used as a favoring agent and also used as a Thai traditional medicine to treat flatulence. Objective: To analyze active ingredients, total phenolic and total flavonoid contents, anti-inflammat...

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Main Authors: Nittaya Chansiw, Kamonnaree Chotinantakul, Somdet Srichairatanakool
Format: Journal
Published: 2019
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/63668
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spelling th-cmuir.6653943832-636682019-03-18T02:25:35Z Anti-inflammatory and antioxidant activities of the extracts from leaves and stems of Polygonum odoratum Lour Nittaya Chansiw Kamonnaree Chotinantakul Somdet Srichairatanakool Immunology and Microbiology Medicine Pharmacology, Toxicology and Pharmaceutics © 2019 Bentham Science Publishers. Background: Polygonum odoratum is an indigenous vegetable that has been used as a favoring agent and also used as a Thai traditional medicine to treat flatulence. Objective: To analyze active ingredients, total phenolic and total flavonoid contents, anti-inflammatory and antioxidant activities from leaf and stem extracts of P. odoratum. Methods: Leaves and stems were dried and extracted by using methanol, dichloromethane and water for obtaining Methanolic Leaf Extract (MLE), Methanolic Stem Extract (MTE), Dichloromethane Leaf Extract (DLE), Dichloromethane Stem Extract (DTE), Water Leaf Extract (WLE) and Water Stem Extract (WTE). The extracts were quantified for total phenolic and total flavonoid contents by spectrophotometry and active compounds were analyzed by using GC-MS. Antioxidant activity was determined by ABTS and DPPH radicals scavenging assays. Anti-inflammatory activity was tested by the inhibition of nitric oxide production in RAW 264.7 macrophage cells induced by lipopolysaccharide. Results: The DLE exhibited the most potent anti-inflammatory effect by inhibiting nitric oxide production in a concentration-dependent manner (IC 50 = 53.75+0.72 µg/mL). MLE exhibited strong antioxidant activity and contained the highest concentration of phenolic compounds (52.59+0.58 mg gallic acid equivalent/g extract) and flavonoid (19.97+0.11 mg quercetin equivalent/g extract). E-15-Heptadecenal and 3, 7, 11, 15-tetramethyl-2-hexadecen-1-ol were found predominantly in the methanol extracts. Conclusion: The leaf extract of P. odoratum showed potent anti-inflammatory and antioxidant activities, mediated by DLE and MLE, respectively. 2019-03-18T02:23:27Z 2019-03-18T02:23:27Z 2019-01-01 Journal 1875614X 18715230 2-s2.0-85061854545 10.2174/1871523017666181109144548 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061854545&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/63668
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Microbiology
Medicine
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Immunology and Microbiology
Medicine
Pharmacology, Toxicology and Pharmaceutics
Nittaya Chansiw
Kamonnaree Chotinantakul
Somdet Srichairatanakool
Anti-inflammatory and antioxidant activities of the extracts from leaves and stems of Polygonum odoratum Lour
description © 2019 Bentham Science Publishers. Background: Polygonum odoratum is an indigenous vegetable that has been used as a favoring agent and also used as a Thai traditional medicine to treat flatulence. Objective: To analyze active ingredients, total phenolic and total flavonoid contents, anti-inflammatory and antioxidant activities from leaf and stem extracts of P. odoratum. Methods: Leaves and stems were dried and extracted by using methanol, dichloromethane and water for obtaining Methanolic Leaf Extract (MLE), Methanolic Stem Extract (MTE), Dichloromethane Leaf Extract (DLE), Dichloromethane Stem Extract (DTE), Water Leaf Extract (WLE) and Water Stem Extract (WTE). The extracts were quantified for total phenolic and total flavonoid contents by spectrophotometry and active compounds were analyzed by using GC-MS. Antioxidant activity was determined by ABTS and DPPH radicals scavenging assays. Anti-inflammatory activity was tested by the inhibition of nitric oxide production in RAW 264.7 macrophage cells induced by lipopolysaccharide. Results: The DLE exhibited the most potent anti-inflammatory effect by inhibiting nitric oxide production in a concentration-dependent manner (IC 50 = 53.75+0.72 µg/mL). MLE exhibited strong antioxidant activity and contained the highest concentration of phenolic compounds (52.59+0.58 mg gallic acid equivalent/g extract) and flavonoid (19.97+0.11 mg quercetin equivalent/g extract). E-15-Heptadecenal and 3, 7, 11, 15-tetramethyl-2-hexadecen-1-ol were found predominantly in the methanol extracts. Conclusion: The leaf extract of P. odoratum showed potent anti-inflammatory and antioxidant activities, mediated by DLE and MLE, respectively.
format Journal
author Nittaya Chansiw
Kamonnaree Chotinantakul
Somdet Srichairatanakool
author_facet Nittaya Chansiw
Kamonnaree Chotinantakul
Somdet Srichairatanakool
author_sort Nittaya Chansiw
title Anti-inflammatory and antioxidant activities of the extracts from leaves and stems of Polygonum odoratum Lour
title_short Anti-inflammatory and antioxidant activities of the extracts from leaves and stems of Polygonum odoratum Lour
title_full Anti-inflammatory and antioxidant activities of the extracts from leaves and stems of Polygonum odoratum Lour
title_fullStr Anti-inflammatory and antioxidant activities of the extracts from leaves and stems of Polygonum odoratum Lour
title_full_unstemmed Anti-inflammatory and antioxidant activities of the extracts from leaves and stems of Polygonum odoratum Lour
title_sort anti-inflammatory and antioxidant activities of the extracts from leaves and stems of polygonum odoratum lour
publishDate 2019
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061854545&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/63668
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