Combined exercise and calorie restriction therapies restore contractile and mitochondrial functions in skeletal muscle of obese–insulin resistant rats
© 2018 Elsevier Inc. Objectives: A combined exercise training and calorie-restriction program is the mainstream treatment of obesity. However, the effect of the dual-action program on mitochondrial function in skeletal muscles has not yet been clarified. The aim of this study was to determine if the...
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Main Authors: | , , , , , , , , |
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Format: | Journal |
Published: |
2019
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Subjects: | |
Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85062468182&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/63690 |
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Institution: | Chiang Mai University |
Summary: | © 2018 Elsevier Inc. Objectives: A combined exercise training and calorie-restriction program is the mainstream treatment of obesity. However, the effect of the dual-action program on mitochondrial function in skeletal muscles has not yet been clarified. The aim of this study was to determine if the combined program, rather than a single program, restored both lost muscle activity and mitochondrial function in obesity. Methods: The study included 30 female Wistar rats. Six rats fed a normal diet for 27 wk were used as the control group. The remaining 24 rats were fed a high-fat diet (HFD) for 27 wk. At week 20, the HFD rats were divided into the following four groups: sedentary lifestyle, endurance exercise five times per week, 60% of calorie restriction (CR) per day, and combined exercise training and CR. All conditions were maintained for 7 wk. Results: We found that HFD-fed rats without therapy developed obese insulin resistance (IR) and impaired function of skeletal muscles. Skeletal muscles of the HFD-fed rats without therapy also exhibited early fatigability; impaired mitochondrial function, as indicated by increased reactive oxygen species production, membrane depolarization, and swelling; reduced mitochondrial dynamics as indicated by increased phosphorylation of DRP1 and decreased MFN2 expression; diminished mitochondrial biogenesis, as shown by decreased PGC1α and CPT1 expression; and increased apoptosis. Both exercise and CR in HFD-fed rats equally attenuated the impairment of muscle functions. However, combined therapies in HFD-fed rats restored functions of skeletal muscles. Conclusions: These findings reinforce the synergistic beneficial effects of combined exercise and CR on skeletal muscles of HFD-fed rats. |
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