Combined exercise and calorie restriction therapies restore contractile and mitochondrial functions in skeletal muscle of obese–insulin resistant rats

© 2018 Elsevier Inc. Objectives: A combined exercise training and calorie-restriction program is the mainstream treatment of obesity. However, the effect of the dual-action program on mitochondrial function in skeletal muscles has not yet been clarified. The aim of this study was to determine if the...

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Main Authors: Sintip Pattanakuhar, Wissuta Sutham, Jirapas Sripetchwandee, Wanitchaya Minta, Duangkamol Mantor, Siripong Palee, Wasana Pratchayasakul, Nipon Chattipakorn, Siriporn C. Chattipakorn
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Published: 2019
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/63690
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spelling th-cmuir.6653943832-636902019-03-18T02:25:18Z Combined exercise and calorie restriction therapies restore contractile and mitochondrial functions in skeletal muscle of obese–insulin resistant rats Sintip Pattanakuhar Wissuta Sutham Jirapas Sripetchwandee Wanitchaya Minta Duangkamol Mantor Siripong Palee Wasana Pratchayasakul Nipon Chattipakorn Siriporn C. Chattipakorn Medicine Nursing © 2018 Elsevier Inc. Objectives: A combined exercise training and calorie-restriction program is the mainstream treatment of obesity. However, the effect of the dual-action program on mitochondrial function in skeletal muscles has not yet been clarified. The aim of this study was to determine if the combined program, rather than a single program, restored both lost muscle activity and mitochondrial function in obesity. Methods: The study included 30 female Wistar rats. Six rats fed a normal diet for 27 wk were used as the control group. The remaining 24 rats were fed a high-fat diet (HFD) for 27 wk. At week 20, the HFD rats were divided into the following four groups: sedentary lifestyle, endurance exercise five times per week, 60% of calorie restriction (CR) per day, and combined exercise training and CR. All conditions were maintained for 7 wk. Results: We found that HFD-fed rats without therapy developed obese insulin resistance (IR) and impaired function of skeletal muscles. Skeletal muscles of the HFD-fed rats without therapy also exhibited early fatigability; impaired mitochondrial function, as indicated by increased reactive oxygen species production, membrane depolarization, and swelling; reduced mitochondrial dynamics as indicated by increased phosphorylation of DRP1 and decreased MFN2 expression; diminished mitochondrial biogenesis, as shown by decreased PGC1α and CPT1 expression; and increased apoptosis. Both exercise and CR in HFD-fed rats equally attenuated the impairment of muscle functions. However, combined therapies in HFD-fed rats restored functions of skeletal muscles. Conclusions: These findings reinforce the synergistic beneficial effects of combined exercise and CR on skeletal muscles of HFD-fed rats. 2019-03-18T02:24:09Z 2019-03-18T02:24:09Z 2019-06-01 Journal 18731244 08999007 2-s2.0-85062468182 10.1016/j.nut.2018.11.031 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85062468182&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/63690
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
Nursing
spellingShingle Medicine
Nursing
Sintip Pattanakuhar
Wissuta Sutham
Jirapas Sripetchwandee
Wanitchaya Minta
Duangkamol Mantor
Siripong Palee
Wasana Pratchayasakul
Nipon Chattipakorn
Siriporn C. Chattipakorn
Combined exercise and calorie restriction therapies restore contractile and mitochondrial functions in skeletal muscle of obese–insulin resistant rats
description © 2018 Elsevier Inc. Objectives: A combined exercise training and calorie-restriction program is the mainstream treatment of obesity. However, the effect of the dual-action program on mitochondrial function in skeletal muscles has not yet been clarified. The aim of this study was to determine if the combined program, rather than a single program, restored both lost muscle activity and mitochondrial function in obesity. Methods: The study included 30 female Wistar rats. Six rats fed a normal diet for 27 wk were used as the control group. The remaining 24 rats were fed a high-fat diet (HFD) for 27 wk. At week 20, the HFD rats were divided into the following four groups: sedentary lifestyle, endurance exercise five times per week, 60% of calorie restriction (CR) per day, and combined exercise training and CR. All conditions were maintained for 7 wk. Results: We found that HFD-fed rats without therapy developed obese insulin resistance (IR) and impaired function of skeletal muscles. Skeletal muscles of the HFD-fed rats without therapy also exhibited early fatigability; impaired mitochondrial function, as indicated by increased reactive oxygen species production, membrane depolarization, and swelling; reduced mitochondrial dynamics as indicated by increased phosphorylation of DRP1 and decreased MFN2 expression; diminished mitochondrial biogenesis, as shown by decreased PGC1α and CPT1 expression; and increased apoptosis. Both exercise and CR in HFD-fed rats equally attenuated the impairment of muscle functions. However, combined therapies in HFD-fed rats restored functions of skeletal muscles. Conclusions: These findings reinforce the synergistic beneficial effects of combined exercise and CR on skeletal muscles of HFD-fed rats.
format Journal
author Sintip Pattanakuhar
Wissuta Sutham
Jirapas Sripetchwandee
Wanitchaya Minta
Duangkamol Mantor
Siripong Palee
Wasana Pratchayasakul
Nipon Chattipakorn
Siriporn C. Chattipakorn
author_facet Sintip Pattanakuhar
Wissuta Sutham
Jirapas Sripetchwandee
Wanitchaya Minta
Duangkamol Mantor
Siripong Palee
Wasana Pratchayasakul
Nipon Chattipakorn
Siriporn C. Chattipakorn
author_sort Sintip Pattanakuhar
title Combined exercise and calorie restriction therapies restore contractile and mitochondrial functions in skeletal muscle of obese–insulin resistant rats
title_short Combined exercise and calorie restriction therapies restore contractile and mitochondrial functions in skeletal muscle of obese–insulin resistant rats
title_full Combined exercise and calorie restriction therapies restore contractile and mitochondrial functions in skeletal muscle of obese–insulin resistant rats
title_fullStr Combined exercise and calorie restriction therapies restore contractile and mitochondrial functions in skeletal muscle of obese–insulin resistant rats
title_full_unstemmed Combined exercise and calorie restriction therapies restore contractile and mitochondrial functions in skeletal muscle of obese–insulin resistant rats
title_sort combined exercise and calorie restriction therapies restore contractile and mitochondrial functions in skeletal muscle of obese–insulin resistant rats
publishDate 2019
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85062468182&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/63690
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