Co-production of Hydrogen and Ethanol of Escherichia coli SS1 Isolate
Simultaneous production of hydrogen and ethanol from waste materials has potential for the development of a more cost-effective biofuels generation process. This study aimed to conduct glycerol fermentation using Escherichia coli SS1 to establish its hydrogen and ethanol co-production profile. Anaer...
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| Main Authors: | , , , , , |
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| Format: | บทความวารสาร |
| Language: | English |
| Published: |
Science Faculty of Chiang Mai University
2019
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| Online Access: | http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8266 http://cmuir.cmu.ac.th/jspui/handle/6653943832/63900 |
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| Institution: | Chiang Mai University |
| Language: | English |
| Summary: | Simultaneous production of hydrogen and ethanol from waste materials has potential for the development of a more cost-effective biofuels generation process. This study aimed to conduct glycerol fermentation using Escherichia coli SS1 to establish its hydrogen and ethanol co-production profile. Anaerobic fermentation was performed at 37°C with different concentrations of glycerol as a substrate. E. coli SS1 had exponential growth within 24 h (OD600 of 1.6), and hydrogen and ethanol were produced in abundance within 48 h of fermentation. Fermentation using 10 g/l of glycerol achieved the highest yield, 0.57 mol of hydrogen and 0.88 mol of ethanol per mol of glycerol. The highest hydrogen productivity (1.85 mmol/l/h) and ethanol productivity (3.13 mmol/l/h) were obtained at 45 g/l of glycerol. This report provides the complete data set for hydrogen and ethanol co-production yield and productivity by the wild-type E. coli SS1 and serves as a useful reference for other researchers working on the co-production of hydrogen and ethanol. |
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