Co-production of Hydrogen and Ethanol of Escherichia coli SS1 Isolate

• Main Authors: Chiu-Shyan Soo, Wai-Sum Yap, Wei-Min Hon, Norhayati Ramli, Umi Kalsom Md Shah, Lai-Yee Phang
• Format: บทความวารสาร
• Language: English
• Published: Science Faculty of Chiang Mai University 2019
• Online Access: http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8266; http://cmuir.cmu.ac.th/jspui/handle/6653943832/63900
• Institution: Chiang Mai University

Simultaneous production of hydrogen and ethanol from waste materials has potential for the development of a more cost-effective biofuels generation process. This study aimed to conduct glycerol fermentation using Escherichia coli SS1 to establish its hydrogen and ethanol co-production profile. Anaerobic fermentation was performed at 37°C with different concentrations of glycerol as a substrate. E. coli SS1 had exponential growth within 24 h (OD600 of 1.6), and hydrogen and ethanol were produced in abundance within 48 h of fermentation. Fermentation using 10 g/l of glycerol achieved the highest yield, 0.57 mol of hydrogen and 0.88 mol of ethanol per mol of glycerol. The highest hydrogen productivity (1.85 mmol/l/h) and ethanol productivity (3.13 mmol/l/h) were obtained at 45 g/l of glycerol. This report provides the complete data set for hydrogen and ethanol co-production yield and productivity by the wild-type E. coli SS1 and serves as a useful reference for other researchers working on the co-production of hydrogen and ethanol.