Phytochemical Constituents of Thai Dark Purple Glutinous Rice Bran Extract [Cultivar Luem Pua (Oryza sativa L.)]

The isolation and identification of phytochemical constituents in the bran of dark-purple glutinous rice cultivar Luem Pua, was conducted. Luem Pua rice bran was extracted with hexane and methanol. The investigation of phytochemicals in the hexane extract was carried out after the fats were eliminat...

Full description

Saved in:
Bibliographic Details
Main Authors: Pattawat Seekhaw, Sugunya Mahatheeranont, Phumon Sookwong, Suwaporn Luangkamin, Acharaporn Na Lampang Neonplab, Pakawan Puangsombat
Format: บทความวารสาร
Language:English
Published: Science Faculty of Chiang Mai University 2019
Online Access:http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9146
http://cmuir.cmu.ac.th/jspui/handle/6653943832/64136
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
Language: English
id th-cmuir.6653943832-64136
record_format dspace
spelling th-cmuir.6653943832-641362019-05-07T09:59:49Z Phytochemical Constituents of Thai Dark Purple Glutinous Rice Bran Extract [Cultivar Luem Pua (Oryza sativa L.)] Pattawat Seekhaw Sugunya Mahatheeranont Phumon Sookwong Suwaporn Luangkamin Acharaporn Na Lampang Neonplab Pakawan Puangsombat The isolation and identification of phytochemical constituents in the bran of dark-purple glutinous rice cultivar Luem Pua, was conducted. Luem Pua rice bran was extracted with hexane and methanol. The investigation of phytochemicals in the hexane extract was carried out after the fats were eliminated by saponification reaction. The isolation of the unsaponified fraction by reverse-phase high-performance liquid chromatography resulted in four sub-fractions, from which seven sterols and two triterpenoids were identified. The structures of all identified compounds were confirmed by gas chromatography-mass spectrometry and proton nuclear magnetic resonance spectroscopy. The chemical constituents in the methanol extract were also investigated using column chromatography and semi-preparative HPLC. The chemical composition of each fractions were analyzed by liquid chromatography coupled with tandem mass spectrometry via electrospray ionization mode (LC-MS/MS), together with comparison with literature data. This resulted in the identification of cyanidin-3-O-glucoside, a major anthocyanin contributing to the dark-purple color and the highly oxidative property of the rice, along with six phenolic compounds and eight flavonoids. The findings from this work helps support the potentials of Luem Pua rice for further applications in either health or pharmaceutical products. 2019-05-07T09:59:49Z 2019-05-07T09:59:49Z 2018 บทความวารสาร 0125-2526 http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9146 http://cmuir.cmu.ac.th/jspui/handle/6653943832/64136 Eng Science Faculty of Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description The isolation and identification of phytochemical constituents in the bran of dark-purple glutinous rice cultivar Luem Pua, was conducted. Luem Pua rice bran was extracted with hexane and methanol. The investigation of phytochemicals in the hexane extract was carried out after the fats were eliminated by saponification reaction. The isolation of the unsaponified fraction by reverse-phase high-performance liquid chromatography resulted in four sub-fractions, from which seven sterols and two triterpenoids were identified. The structures of all identified compounds were confirmed by gas chromatography-mass spectrometry and proton nuclear magnetic resonance spectroscopy. The chemical constituents in the methanol extract were also investigated using column chromatography and semi-preparative HPLC. The chemical composition of each fractions were analyzed by liquid chromatography coupled with tandem mass spectrometry via electrospray ionization mode (LC-MS/MS), together with comparison with literature data. This resulted in the identification of cyanidin-3-O-glucoside, a major anthocyanin contributing to the dark-purple color and the highly oxidative property of the rice, along with six phenolic compounds and eight flavonoids. The findings from this work helps support the potentials of Luem Pua rice for further applications in either health or pharmaceutical products.
format บทความวารสาร
author Pattawat Seekhaw
Sugunya Mahatheeranont
Phumon Sookwong
Suwaporn Luangkamin
Acharaporn Na Lampang Neonplab
Pakawan Puangsombat
spellingShingle Pattawat Seekhaw
Sugunya Mahatheeranont
Phumon Sookwong
Suwaporn Luangkamin
Acharaporn Na Lampang Neonplab
Pakawan Puangsombat
Phytochemical Constituents of Thai Dark Purple Glutinous Rice Bran Extract [Cultivar Luem Pua (Oryza sativa L.)]
author_facet Pattawat Seekhaw
Sugunya Mahatheeranont
Phumon Sookwong
Suwaporn Luangkamin
Acharaporn Na Lampang Neonplab
Pakawan Puangsombat
author_sort Pattawat Seekhaw
title Phytochemical Constituents of Thai Dark Purple Glutinous Rice Bran Extract [Cultivar Luem Pua (Oryza sativa L.)]
title_short Phytochemical Constituents of Thai Dark Purple Glutinous Rice Bran Extract [Cultivar Luem Pua (Oryza sativa L.)]
title_full Phytochemical Constituents of Thai Dark Purple Glutinous Rice Bran Extract [Cultivar Luem Pua (Oryza sativa L.)]
title_fullStr Phytochemical Constituents of Thai Dark Purple Glutinous Rice Bran Extract [Cultivar Luem Pua (Oryza sativa L.)]
title_full_unstemmed Phytochemical Constituents of Thai Dark Purple Glutinous Rice Bran Extract [Cultivar Luem Pua (Oryza sativa L.)]
title_sort phytochemical constituents of thai dark purple glutinous rice bran extract [cultivar luem pua (oryza sativa l.)]
publisher Science Faculty of Chiang Mai University
publishDate 2019
url http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9146
http://cmuir.cmu.ac.th/jspui/handle/6653943832/64136
_version_ 1681426025301409792