Effect of acidic amino acid substitution at the pr-M junction of dengue type 2 virus on prM cleavage and virus replication
Chiang Mai Medical Journal (Formerly Chiang Mai Medical Bulletin) is an official journal of the Faculty of Medicine, Chiang Mai University. It accepts original papers on clinical and experimental research that are pertinent in the biomedical sciences. The Journal is published 4 issues/year (i.e., Ma...
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Faculty of Medicine, Chiang Mai University
2019
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| Online Access: | https://www.tci-thaijo.org/index.php/CMMJ-MedCMJ/article/view/87750/69221 http://cmuir.cmu.ac.th/jspui/handle/6653943832/65026 |
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th-cmuir.6653943832-650262019-05-07T10:02:37Z Effect of acidic amino acid substitution at the pr-M junction of dengue type 2 virus on prM cleavage and virus replication ผลของการแทนที่กรดอะมิโนที่มีคุณสมบัติเป็นกรดที่บริเวณรอยต่อของโปรตีน pr-M ของเชื้อไวรัสเด็งกี่ ไทป์ 2 ต่อระดับการตัดโปรตีน prM และการเพิ่มจํานวนของเชื้อ Rungtawan Sriburi Tassanee Rattanapak Tassanee Rattanapak Chiang Mai Medical Journal (Formerly Chiang Mai Medical Bulletin) is an official journal of the Faculty of Medicine, Chiang Mai University. It accepts original papers on clinical and experimental research that are pertinent in the biomedical sciences. The Journal is published 4 issues/year (i.e., Mar, Jun, Sep, and Dec). Cleavage by furin of the envelope glycoprotein, prM, is required for maturation and infectivity of the dengue virus. Highly conserved acidic amino acid, aspartate in DENV-1 and -3 or glutamate in DENV-2 and -4, at a P3 position proximal to the furin cleavage site, infl uences the level of prM cleavage during virus replication. However, the signifi cance of this inter-serotypic variation of P3 acidic residue has not been elucidated. The aim of this study was to defi ne the effect of acidic amino acid variation at this P3 position in the pr-M junction on the extent of prM cleavage and virus replication. The mutant virus, strain E203D, containing a P3 glutamate-to-aspartate substitution was generated by site-directed mutagenesis of a full-length cDNA clone of the dengue serotype 2 virus, strain 16681. The level of prM cleavage, as determined by the metabolic labeling method employing 35S-Cys/Met, in the strain E203D was comparable to the parent virus. When virus replicative ability was assessed in single and multi-step multiplication studies, no difference existed in the kinetics of replication between the strain E203D and the parent virus. A glutamate-to-aspartate substitution in prM at the polyprotein position 203 does not appear to affect the level of prM cleavage or viral replication in the dengue serotype 2 virus. 2019-05-07T10:02:37Z 2019-05-07T10:02:37Z 2016 บทความวารสาร 0125-5983 https://www.tci-thaijo.org/index.php/CMMJ-MedCMJ/article/view/87750/69221 http://cmuir.cmu.ac.th/jspui/handle/6653943832/65026 Eng Faculty of Medicine, Chiang Mai University |
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| description |
Chiang Mai Medical Journal (Formerly Chiang Mai Medical Bulletin) is an official journal of the Faculty of Medicine, Chiang Mai University. It accepts original papers on clinical and experimental research that are pertinent in the biomedical sciences. The Journal is published 4 issues/year (i.e., Mar, Jun, Sep, and Dec). |
| format |
บทความวารสาร |
| author |
Rungtawan Sriburi Tassanee Rattanapak Tassanee Rattanapak |
| spellingShingle |
Rungtawan Sriburi Tassanee Rattanapak Tassanee Rattanapak Effect of acidic amino acid substitution at the pr-M junction of dengue type 2 virus on prM cleavage and virus replication |
| author_facet |
Rungtawan Sriburi Tassanee Rattanapak Tassanee Rattanapak |
| author_sort |
Rungtawan Sriburi |
| title |
Effect of acidic amino acid substitution at the pr-M junction of dengue type 2 virus on prM cleavage and virus replication |
| title_short |
Effect of acidic amino acid substitution at the pr-M junction of dengue type 2 virus on prM cleavage and virus replication |
| title_full |
Effect of acidic amino acid substitution at the pr-M junction of dengue type 2 virus on prM cleavage and virus replication |
| title_fullStr |
Effect of acidic amino acid substitution at the pr-M junction of dengue type 2 virus on prM cleavage and virus replication |
| title_full_unstemmed |
Effect of acidic amino acid substitution at the pr-M junction of dengue type 2 virus on prM cleavage and virus replication |
| title_sort |
effect of acidic amino acid substitution at the pr-m junction of dengue type 2 virus on prm cleavage and virus replication |
| publisher |
Faculty of Medicine, Chiang Mai University |
| publishDate |
2019 |
| url |
https://www.tci-thaijo.org/index.php/CMMJ-MedCMJ/article/view/87750/69221 http://cmuir.cmu.ac.th/jspui/handle/6653943832/65026 |
| _version_ |
1681426191922233344 |