Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune

© 2019 Elsevier Ltd Polysaccharides were extracted from Schizophyllum commune (a common mushroom) and their structural and immune-enhancing properties were investigated. Crude and fractions (F1 and F2) were composed of sugars (50.3–82.8%), proteins (1.46–20.1%), and sulfates (1.33–7.01%). Monosaccha...

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Main Authors: Khamphone Yelithao, Utoomporn Surayot, Changsheng Lee, Subramanian Palanisamy, Narayanasamy Marimuthu Prabhu, Ju Hun Lee, Sang Guan You
Format: Journal
Published: 2019
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/65475
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-654752019-08-05T04:38:30Z Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune Khamphone Yelithao Utoomporn Surayot Changsheng Lee Subramanian Palanisamy Narayanasamy Marimuthu Prabhu Ju Hun Lee Sang Guan You Chemistry Materials Science © 2019 Elsevier Ltd Polysaccharides were extracted from Schizophyllum commune (a common mushroom) and their structural and immune-enhancing properties were investigated. Crude and fractions (F1 and F2) were composed of sugars (50.3–82.8%), proteins (1.46–20.1%), and sulfates (1.33–7.01%). Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose. Their immune-enhancing activities were tested using RAW264.7 cells. Proliferation activity of RAW264.7 cells was over 100% after treatment with these polysaccharides. In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides. In addition, RAW264.7 cells were activated mainly through CR-3 and TLR-4 receptors. The backbone of F2 with excellent immune-enhancing activity was mainly linked by (1→3)-linked-mannopyranosyl and (1→2,3)-linked-mannopyranosyl residues. 2019-08-05T04:33:55Z 2019-08-05T04:33:55Z 2019-08-15 Journal 01448617 2-s2.0-85064922641 10.1016/j.carbpol.2019.04.057 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85064922641&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/65475
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Chemistry
Materials Science
spellingShingle Chemistry
Materials Science
Khamphone Yelithao
Utoomporn Surayot
Changsheng Lee
Subramanian Palanisamy
Narayanasamy Marimuthu Prabhu
Ju Hun Lee
Sang Guan You
Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune
description © 2019 Elsevier Ltd Polysaccharides were extracted from Schizophyllum commune (a common mushroom) and their structural and immune-enhancing properties were investigated. Crude and fractions (F1 and F2) were composed of sugars (50.3–82.8%), proteins (1.46–20.1%), and sulfates (1.33–7.01%). Monosaccharide compositions of Cr and F1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose. Their immune-enhancing activities were tested using RAW264.7 cells. Proliferation activity of RAW264.7 cells was over 100% after treatment with these polysaccharides. In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-κB) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides. In addition, RAW264.7 cells were activated mainly through CR-3 and TLR-4 receptors. The backbone of F2 with excellent immune-enhancing activity was mainly linked by (1→3)-linked-mannopyranosyl and (1→2,3)-linked-mannopyranosyl residues.
format Journal
author Khamphone Yelithao
Utoomporn Surayot
Changsheng Lee
Subramanian Palanisamy
Narayanasamy Marimuthu Prabhu
Ju Hun Lee
Sang Guan You
author_facet Khamphone Yelithao
Utoomporn Surayot
Changsheng Lee
Subramanian Palanisamy
Narayanasamy Marimuthu Prabhu
Ju Hun Lee
Sang Guan You
author_sort Khamphone Yelithao
title Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune
title_short Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune
title_full Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune
title_fullStr Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune
title_full_unstemmed Studies on structural properties and immune-enhancing activities of glycomannans from Schizophyllum commune
title_sort studies on structural properties and immune-enhancing activities of glycomannans from schizophyllum commune
publishDate 2019
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85064922641&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/65475
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