Protease-producing bacteria from soil in Nasinuan community forest, Mahasarakham Province, Thailand

Oriental Scientific Publishing Company © 2019 The current work aimed to screen for and identify protease-producing bacteria from the untapped resource Nasinuan forest, Kantarawichai District, Mahasarakham Province, Thailand. Nineteen bacterial isolates with protease-producing capacity on 1% skimmed...

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Main Authors: Vijitra Luang-In, Manatchanok Yotchaisarn, Worachot Saengha, Piyachat Udomwong, Sirirat Deeseenthum, Kedsukon Maneewan
Format: Journal
Published: 2019
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/65861
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-658612019-08-05T04:42:54Z Protease-producing bacteria from soil in Nasinuan community forest, Mahasarakham Province, Thailand Vijitra Luang-In Manatchanok Yotchaisarn Worachot Saengha Piyachat Udomwong Sirirat Deeseenthum Kedsukon Maneewan Pharmacology, Toxicology and Pharmaceutics Oriental Scientific Publishing Company © 2019 The current work aimed to screen for and identify protease-producing bacteria from the untapped resource Nasinuan forest, Kantarawichai District, Mahasarakham Province, Thailand. Nineteen bacterial isolates with protease-producing capacity on 1% skimmed milk agar were identified using 16S rRNA sequencing. Seventeen bacteria were gram-positive, rod shaped and identified as Bacillus spp. and only two bacteria were identified as Enterobacter sp. and Staphylococcus cohnii. Their closest relatives were found in India, Oman, Italy, Indonesia, Malaysia, China and USA. The top six highest halo: colony ratios from pure isolates were ranked in the following order: 1.2PT1 (2.43) >1.2PT2 (2.23) >2.2PT3(2.21) > 2.1PT3 (2.17) >2.3PT3(2.16) >2.4PT1 (2.16). Bacillus thuringiensis 2.3PT3 was found to exhibit the highest protease enzyme activity of 3.72 ± 0.08 U/mg protein at the optimal conditions of 65°C and pH 8.0 after 30 min incubation with 1% casein in 0.05 M PBS buffer. This protease–producing bacterial strain might be of great potential for applications in food,agricultural and pharmaceutical industries in Thailand. 2019-08-05T04:42:54Z 2019-08-05T04:42:54Z 2019-01-01 Journal 24562610 09746242 2-s2.0-85069579707 10.13005/bpj/1678 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85069579707&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/65861
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Pharmacology, Toxicology and Pharmaceutics
spellingShingle Pharmacology, Toxicology and Pharmaceutics
Vijitra Luang-In
Manatchanok Yotchaisarn
Worachot Saengha
Piyachat Udomwong
Sirirat Deeseenthum
Kedsukon Maneewan
Protease-producing bacteria from soil in Nasinuan community forest, Mahasarakham Province, Thailand
description Oriental Scientific Publishing Company © 2019 The current work aimed to screen for and identify protease-producing bacteria from the untapped resource Nasinuan forest, Kantarawichai District, Mahasarakham Province, Thailand. Nineteen bacterial isolates with protease-producing capacity on 1% skimmed milk agar were identified using 16S rRNA sequencing. Seventeen bacteria were gram-positive, rod shaped and identified as Bacillus spp. and only two bacteria were identified as Enterobacter sp. and Staphylococcus cohnii. Their closest relatives were found in India, Oman, Italy, Indonesia, Malaysia, China and USA. The top six highest halo: colony ratios from pure isolates were ranked in the following order: 1.2PT1 (2.43) >1.2PT2 (2.23) >2.2PT3(2.21) > 2.1PT3 (2.17) >2.3PT3(2.16) >2.4PT1 (2.16). Bacillus thuringiensis 2.3PT3 was found to exhibit the highest protease enzyme activity of 3.72 ± 0.08 U/mg protein at the optimal conditions of 65°C and pH 8.0 after 30 min incubation with 1% casein in 0.05 M PBS buffer. This protease–producing bacterial strain might be of great potential for applications in food,agricultural and pharmaceutical industries in Thailand.
format Journal
author Vijitra Luang-In
Manatchanok Yotchaisarn
Worachot Saengha
Piyachat Udomwong
Sirirat Deeseenthum
Kedsukon Maneewan
author_facet Vijitra Luang-In
Manatchanok Yotchaisarn
Worachot Saengha
Piyachat Udomwong
Sirirat Deeseenthum
Kedsukon Maneewan
author_sort Vijitra Luang-In
title Protease-producing bacteria from soil in Nasinuan community forest, Mahasarakham Province, Thailand
title_short Protease-producing bacteria from soil in Nasinuan community forest, Mahasarakham Province, Thailand
title_full Protease-producing bacteria from soil in Nasinuan community forest, Mahasarakham Province, Thailand
title_fullStr Protease-producing bacteria from soil in Nasinuan community forest, Mahasarakham Province, Thailand
title_full_unstemmed Protease-producing bacteria from soil in Nasinuan community forest, Mahasarakham Province, Thailand
title_sort protease-producing bacteria from soil in nasinuan community forest, mahasarakham province, thailand
publishDate 2019
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85069579707&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/65861
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