Medium Optimization for b-Galactosidase Production by a Thermotolerant Yeast

• Main Authors: Sirinda Am-aiam, Chartchai Khanongnuch
• Language: English
• Published: Science Faculty of Chiang Mai University 2019
• Subjects: b-galactosidase; thermotolerant yeast; medium optimization; 2-level factorial experimental design; central composite design
• Online Access: http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=6238; http://cmuir.cmu.ac.th/jspui/handle/6653943832/66158
• Institution: Chiang Mai University

Kluyveromyces sp. CK8 was previously isolated from rotten fruit in northern Thailand and found to be capable of growing at 45°C. This research aimed to identify the yeast isolate and to optimize its production of b-galactosidase in synthetic medium using lactose as the sole carbon source. Molecular...