Statistical Optimization for the Production of D-Mannose Isomerase by Saccharothrix sp. CMU-K747 Using Response Surface Methodology

• Main Authors: ApiradeeSiangsuepchart", SaisamornLumyong
• Language: English
• Published: Science Faculty of Chiang Mai University 2019
• Subjects: Response surface methodology; Medium optimization; D-Mannose isomerase production; Central composite design; Saccharothrixsp. CMU-K747
• Online Access: http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=5513; http://cmuir.cmu.ac.th/jspui/handle/6653943832/66825
• Institution: Chiang Mai University

Response surface methodology (RSM) and central composite design (CCD) were employed to find the optimal fermentation medium for producing intracellular D-mannose isomerase (D-MI) using Saccharothrix sp. CMU-K747. The two variables involved in this study were D-mannose and soybean meal. The statistical analysis of the results showed that, only D-mannose had a significant effect on D-MI production. The optimized medium containing (% w/v) D-mannose, 1.3 and soybean meal, 0.055 resulted in 1.32-fold enhanced in D-MI (1.638 Unit/ml) production compared to the initial level (1.243 Unit/ml). In addition, the effects of incubation period, pH and temperature of D-MI production in the optimized medium composition were also described. This present study is the first report on statistical optimization of medium components for D-MI production and also explored the possibility of using D-MI produced by Saccharothrix sp. CMU-K747 for rare sugar production.