Antioxidant, Anti-tyrosinase, Anti-aging Potentialsand Safety of Arabica Coffee Cherry Extract

used in agriculture; however, there are few reports regarding the application of coffee by-products in cosmetics. Therefore, this study aimed to investigate the antioxidant, anti-tyrosinase and anti-aging activities as well as the toxicity of coffee cherry extracts. The extracts of the coffee cherry...

Full description

Saved in:
Bibliographic Details
Main Authors: Kanokwan Kiattisin, Nutjeera Intasai, Nichcha Nitthikan, Thananya Nantarat, Kuan-Han Lee, Wei-Chao Lin, Shang-Chian Lue, Pimporn Leelapornpisid
Language:English
Published: Science Faculty of Chiang Mai University 2019
Subjects:
Online Access:http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=10398
http://cmuir.cmu.ac.th/jspui/handle/6653943832/66921
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
Language: English
id th-cmuir.6653943832-66921
record_format dspace
spelling th-cmuir.6653943832-669212019-12-03T06:32:24Z Antioxidant, Anti-tyrosinase, Anti-aging Potentialsand Safety of Arabica Coffee Cherry Extract Kanokwan Kiattisin Nutjeera Intasai Nichcha Nitthikan Thananya Nantarat Kuan-Han Lee Wei-Chao Lin Shang-Chian Lue Pimporn Leelapornpisid Coffea arabica coffee cherry antioxidant anti-aging safety testing used in agriculture; however, there are few reports regarding the application of coffee by-products in cosmetics. Therefore, this study aimed to investigate the antioxidant, anti-tyrosinase and anti-aging activities as well as the toxicity of coffee cherry extracts. The extracts of the coffee cherry fruits were obtained by fractionated extraction using hexane, ethyl acetate, and ethanol, respectively. Anti-aging bioactivities were investigated. Results showed that the ethyl acetate extract was shown to contain the highest total phenolic content (301.96 ± 4.05 mg GAE/g extract). It possessed good antioxidant activity according to the DPPH (IC50 value as 35.65 ± 1.35 μg/mL) and FRAP assays (FRAP value as 100.52 ± 0.08 mM FeSO4/g) that the results were higher than caffeine standard. The ethyl acetate extract also showed the highest anti-tyrosinase activity (IC50 values as 0.23 ± 0.01 mg/mL and 0.98 ± 0.02 mg/mL when used L-tyrosine and L-dopa as substrates) when compared with other extracts. However, it showed lower anti-tyrosinase activity than kojic acid and vitamin c. It also demonstrated the highest inhibition against matrix metallopeptidase-2 and hyaluronidase (% inhibition as 25.44 ± 2.1 and 92.95 ± 0.61) when compared with other extracts. Moreover, it was found to be non-toxic to NIH-3T3 cells according to the MTT assay with concentrations 8-800 μg/mL, and an irritation effect was not observed in HET-CAM assay (irritation score was 0.0, at the concentration of 10 mg/mL). Therefore, this coffee cherry extract could be an attractive natural extract for further development of whitening or anti-aging products. Moreover, this can be a way to develop the value of arabica coffee waste into a value product that can be applied in the cosmetics industry 2019-12-03T06:32:24Z 2019-12-03T06:32:24Z 2019 Chiang Mai Journal of Science 46, 5 (Sep 2019), 930 - 945 0125-2526 http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=10398 http://cmuir.cmu.ac.th/jspui/handle/6653943832/66921 Eng Science Faculty of Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
topic Coffea arabica
coffee cherry
antioxidant
anti-aging
safety testing
spellingShingle Coffea arabica
coffee cherry
antioxidant
anti-aging
safety testing
Kanokwan Kiattisin
Nutjeera Intasai
Nichcha Nitthikan
Thananya Nantarat
Kuan-Han Lee
Wei-Chao Lin
Shang-Chian Lue
Pimporn Leelapornpisid
Antioxidant, Anti-tyrosinase, Anti-aging Potentialsand Safety of Arabica Coffee Cherry Extract
description used in agriculture; however, there are few reports regarding the application of coffee by-products in cosmetics. Therefore, this study aimed to investigate the antioxidant, anti-tyrosinase and anti-aging activities as well as the toxicity of coffee cherry extracts. The extracts of the coffee cherry fruits were obtained by fractionated extraction using hexane, ethyl acetate, and ethanol, respectively. Anti-aging bioactivities were investigated. Results showed that the ethyl acetate extract was shown to contain the highest total phenolic content (301.96 ± 4.05 mg GAE/g extract). It possessed good antioxidant activity according to the DPPH (IC50 value as 35.65 ± 1.35 μg/mL) and FRAP assays (FRAP value as 100.52 ± 0.08 mM FeSO4/g) that the results were higher than caffeine standard. The ethyl acetate extract also showed the highest anti-tyrosinase activity (IC50 values as 0.23 ± 0.01 mg/mL and 0.98 ± 0.02 mg/mL when used L-tyrosine and L-dopa as substrates) when compared with other extracts. However, it showed lower anti-tyrosinase activity than kojic acid and vitamin c. It also demonstrated the highest inhibition against matrix metallopeptidase-2 and hyaluronidase (% inhibition as 25.44 ± 2.1 and 92.95 ± 0.61) when compared with other extracts. Moreover, it was found to be non-toxic to NIH-3T3 cells according to the MTT assay with concentrations 8-800 μg/mL, and an irritation effect was not observed in HET-CAM assay (irritation score was 0.0, at the concentration of 10 mg/mL). Therefore, this coffee cherry extract could be an attractive natural extract for further development of whitening or anti-aging products. Moreover, this can be a way to develop the value of arabica coffee waste into a value product that can be applied in the cosmetics industry
author Kanokwan Kiattisin
Nutjeera Intasai
Nichcha Nitthikan
Thananya Nantarat
Kuan-Han Lee
Wei-Chao Lin
Shang-Chian Lue
Pimporn Leelapornpisid
author_facet Kanokwan Kiattisin
Nutjeera Intasai
Nichcha Nitthikan
Thananya Nantarat
Kuan-Han Lee
Wei-Chao Lin
Shang-Chian Lue
Pimporn Leelapornpisid
author_sort Kanokwan Kiattisin
title Antioxidant, Anti-tyrosinase, Anti-aging Potentialsand Safety of Arabica Coffee Cherry Extract
title_short Antioxidant, Anti-tyrosinase, Anti-aging Potentialsand Safety of Arabica Coffee Cherry Extract
title_full Antioxidant, Anti-tyrosinase, Anti-aging Potentialsand Safety of Arabica Coffee Cherry Extract
title_fullStr Antioxidant, Anti-tyrosinase, Anti-aging Potentialsand Safety of Arabica Coffee Cherry Extract
title_full_unstemmed Antioxidant, Anti-tyrosinase, Anti-aging Potentialsand Safety of Arabica Coffee Cherry Extract
title_sort antioxidant, anti-tyrosinase, anti-aging potentialsand safety of arabica coffee cherry extract
publisher Science Faculty of Chiang Mai University
publishDate 2019
url http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=10398
http://cmuir.cmu.ac.th/jspui/handle/6653943832/66921
_version_ 1681426538544758784