Effects of preservation method on fruiting body formation and cordycepin production of Cordyceps militaris culture

© 2019 Elsevier B.V.. All rights reserved. Preservation was investigated of Cordyceps militaris mycelial cultures under eight different methods: freezing at -80°C; chilling at 5°C in 10% (volume per volume; v/v) glycerol; chilling at 5°C on rice grains that had been dried at 35°C, 45°C or 55°C; and...

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Main Authors: Natthapong Singpoonga, Boonsong Sang–on, Peerasak Chaiprasart
Format: Journal
Published: 2020
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/67550
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spelling th-cmuir.6653943832-675502020-04-02T14:55:32Z Effects of preservation method on fruiting body formation and cordycepin production of Cordyceps militaris culture Natthapong Singpoonga Boonsong Sang–on Peerasak Chaiprasart Agricultural and Biological Sciences © 2019 Elsevier B.V.. All rights reserved. Preservation was investigated of Cordyceps militaris mycelial cultures under eight different methods: freezing at -80°C; chilling at 5°C in 10% (volume per volume; v/v) glycerol; chilling at 5°C on rice grains that had been dried at 35°C, 45°C or 55°C; and three subculturings involving holding at 5°C. The cultivation of the original strain before the preservation tests was used as the control. The viability, purity and stability of the mycelia were tested after 4 mth storage. Fruiting body production and physico-chemical properties, together with the cordycepin production were determined. All preservation methods resulted in high viability and purity of the cultures. However, the stability of the preserved culture was significantly (p < 0.05) different among the preservation methods. Cultures chilled at 5°C on rice grains dried at 35°C, 45°C or 55°C and the three subculturing methods affected the size of the colony diameter (p < 0.05). Freezing at -80°C, chilling at 5°C in 10% (v/v) glycerol or keeping on rice grains dried at 35 or 45°C did not affect the number of fruiting bodies produced, fresh weight, firmness, total soluble solids or color (p > 0.05). Cultures frozen at -80°C, chilled at 5°C in 10% (v/v) glycerol, or kept on rice grains dried at 35°C compared to the control did not show any significant (p > 0.05) differences in cordycepin production, while subculturing negatively affected cordycepin production after 4 mth storage. 2020-04-02T14:55:32Z 2020-04-02T14:55:32Z 2019-01-01 Journal 2452316X 24681458 2-s2.0-85072809481 10.34044/j.anres.2019.53.2.03 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85072809481&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/67550
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Natthapong Singpoonga
Boonsong Sang–on
Peerasak Chaiprasart
Effects of preservation method on fruiting body formation and cordycepin production of Cordyceps militaris culture
description © 2019 Elsevier B.V.. All rights reserved. Preservation was investigated of Cordyceps militaris mycelial cultures under eight different methods: freezing at -80°C; chilling at 5°C in 10% (volume per volume; v/v) glycerol; chilling at 5°C on rice grains that had been dried at 35°C, 45°C or 55°C; and three subculturings involving holding at 5°C. The cultivation of the original strain before the preservation tests was used as the control. The viability, purity and stability of the mycelia were tested after 4 mth storage. Fruiting body production and physico-chemical properties, together with the cordycepin production were determined. All preservation methods resulted in high viability and purity of the cultures. However, the stability of the preserved culture was significantly (p < 0.05) different among the preservation methods. Cultures chilled at 5°C on rice grains dried at 35°C, 45°C or 55°C and the three subculturing methods affected the size of the colony diameter (p < 0.05). Freezing at -80°C, chilling at 5°C in 10% (v/v) glycerol or keeping on rice grains dried at 35 or 45°C did not affect the number of fruiting bodies produced, fresh weight, firmness, total soluble solids or color (p > 0.05). Cultures frozen at -80°C, chilled at 5°C in 10% (v/v) glycerol, or kept on rice grains dried at 35°C compared to the control did not show any significant (p > 0.05) differences in cordycepin production, while subculturing negatively affected cordycepin production after 4 mth storage.
format Journal
author Natthapong Singpoonga
Boonsong Sang–on
Peerasak Chaiprasart
author_facet Natthapong Singpoonga
Boonsong Sang–on
Peerasak Chaiprasart
author_sort Natthapong Singpoonga
title Effects of preservation method on fruiting body formation and cordycepin production of Cordyceps militaris culture
title_short Effects of preservation method on fruiting body formation and cordycepin production of Cordyceps militaris culture
title_full Effects of preservation method on fruiting body formation and cordycepin production of Cordyceps militaris culture
title_fullStr Effects of preservation method on fruiting body formation and cordycepin production of Cordyceps militaris culture
title_full_unstemmed Effects of preservation method on fruiting body formation and cordycepin production of Cordyceps militaris culture
title_sort effects of preservation method on fruiting body formation and cordycepin production of cordyceps militaris culture
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85072809481&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/67550
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