Anti-human CD63 monoclonal antibody COS3A upregulates monocyte-induced IL-10 excretion leading to diminution of CD3-mediated T cell response
© 2019 Elsevier Ltd Human CD63 has been reported to play a role either as an inhibitor or as a co-stimulator of T- cell responses, although the mechanism of this is unclear. In this study, an anti-human CD63 monoclonal antibody (mAb) COS3A was used to monitor the role of CD63 in T-cell activation. M...
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th-cmuir.6653943832-676122020-04-02T15:08:11Z Anti-human CD63 monoclonal antibody COS3A upregulates monocyte-induced IL-10 excretion leading to diminution of CD3-mediated T cell response Siriwan Wansook Kodchakorn Mahasongkram Nuttaphol Chruekamlow Supansa Pata Watchara Kasinrerk Panida Khunkaewla Biochemistry, Genetics and Molecular Biology Immunology and Microbiology © 2019 Elsevier Ltd Human CD63 has been reported to play a role either as an inhibitor or as a co-stimulator of T- cell responses, although the mechanism of this is unclear. In this study, an anti-human CD63 monoclonal antibody (mAb) COS3A was used to monitor the role of CD63 in T-cell activation. MAb COS3A could inhibit CD3-mediated T-cell proliferation and CD25 expression in peripheral blood mononuclear cells (PBMCs), used as a study model, but the suppressive effect was not observed when purified T-cells were used instead of PBMCs. The inhibitory phenomenon was associated with downregulation of IL-2 and IFN-γ by T-cells, but upregulation of IL-10 by monocytes. Neutralizing IL-10 with anti-IL-10 mAb improved the T-cell response, indicating the role of IL-10 in T-cell suppression. In this study, monocytes were demonstrated to play a role in impeding T-cell activation by the anti-CD63 mAb COS3A. This is the first evidence that anti-CD63 mAb induces IL-10 secretion by monocytes, which later play a role in T-cell hypo-responsiveness. 2020-04-02T14:56:40Z 2020-04-02T14:56:40Z 2019-10-01 Journal 18729142 01615890 2-s2.0-85072214951 10.1016/j.molimm.2019.09.005 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85072214951&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/67612 |
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Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Siriwan Wansook Kodchakorn Mahasongkram Nuttaphol Chruekamlow Supansa Pata Watchara Kasinrerk Panida Khunkaewla Anti-human CD63 monoclonal antibody COS3A upregulates monocyte-induced IL-10 excretion leading to diminution of CD3-mediated T cell response |
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© 2019 Elsevier Ltd Human CD63 has been reported to play a role either as an inhibitor or as a co-stimulator of T- cell responses, although the mechanism of this is unclear. In this study, an anti-human CD63 monoclonal antibody (mAb) COS3A was used to monitor the role of CD63 in T-cell activation. MAb COS3A could inhibit CD3-mediated T-cell proliferation and CD25 expression in peripheral blood mononuclear cells (PBMCs), used as a study model, but the suppressive effect was not observed when purified T-cells were used instead of PBMCs. The inhibitory phenomenon was associated with downregulation of IL-2 and IFN-γ by T-cells, but upregulation of IL-10 by monocytes. Neutralizing IL-10 with anti-IL-10 mAb improved the T-cell response, indicating the role of IL-10 in T-cell suppression. In this study, monocytes were demonstrated to play a role in impeding T-cell activation by the anti-CD63 mAb COS3A. This is the first evidence that anti-CD63 mAb induces IL-10 secretion by monocytes, which later play a role in T-cell hypo-responsiveness. |
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author |
Siriwan Wansook Kodchakorn Mahasongkram Nuttaphol Chruekamlow Supansa Pata Watchara Kasinrerk Panida Khunkaewla |
author_facet |
Siriwan Wansook Kodchakorn Mahasongkram Nuttaphol Chruekamlow Supansa Pata Watchara Kasinrerk Panida Khunkaewla |
author_sort |
Siriwan Wansook |
title |
Anti-human CD63 monoclonal antibody COS3A upregulates monocyte-induced IL-10 excretion leading to diminution of CD3-mediated T cell response |
title_short |
Anti-human CD63 monoclonal antibody COS3A upregulates monocyte-induced IL-10 excretion leading to diminution of CD3-mediated T cell response |
title_full |
Anti-human CD63 monoclonal antibody COS3A upregulates monocyte-induced IL-10 excretion leading to diminution of CD3-mediated T cell response |
title_fullStr |
Anti-human CD63 monoclonal antibody COS3A upregulates monocyte-induced IL-10 excretion leading to diminution of CD3-mediated T cell response |
title_full_unstemmed |
Anti-human CD63 monoclonal antibody COS3A upregulates monocyte-induced IL-10 excretion leading to diminution of CD3-mediated T cell response |
title_sort |
anti-human cd63 monoclonal antibody cos3a upregulates monocyte-induced il-10 excretion leading to diminution of cd3-mediated t cell response |
publishDate |
2020 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85072214951&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/67612 |
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