Feasibility Study for D-Lactic Acid Production from Thai Rice by Leuconostoc pseudomesenteroides TC49 and D-Lactic Acid Purification

Chiang Mai Journal of Science

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Main Authors: Tasneem Chemama, Chayakorn Pumas, Yingmanee Tragoolpua, Narumol Thongwai
Language:English
Published: Faculty of Science, Chiang Mai University 2020
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Online Access:https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=10913
http://cmuir.cmu.ac.th/jspui/handle/6653943832/68737
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spelling th-cmuir.6653943832-687372020-06-10T07:12:27Z Feasibility Study for D-Lactic Acid Production from Thai Rice by Leuconostoc pseudomesenteroides TC49 and D-Lactic Acid Purification Tasneem Chemama Chayakorn Pumas Yingmanee Tragoolpua Narumol Thongwai Narumol Thongwai - Department of Biology Faculty of Science Chiang Mai University Chiang Mai Journal of Science Two enantiomers of lactic acid, D- and L-forms, are naturally produced by lactic acid bacteria. Generally, L-lactic acid is more abundant than D-lactic acid. Both forms have been importantly used as monomers in bio-plastic industry. In this study, total of 251 lactic acid bacterial strains were isolated and screened from samples collected in southern and northern Thailand. Among these bacteria, only 7 isolates produced D-lactic acid with optical purity ranging from 96.2 to 99.7%. The isolate TC49, identified as Leuconostoc pseudomesenteroides, produced the highest D-lactic acid and was selected for further investigation. When Thai broken rice was used as a raw material for D-lactic acid fermentation by the selected strain, D-lactic acid yield and productivity were 93 ± 0.03 g/l and 1.29 g/l/h, respectively, under the optimal conditions. Upon further recovery and purification by the ultrasonic extraction process, D-lactic acid obtained was 99% optical purity and was structurally examined by 1H-NMR determination. The results indicated the high potential of Leu. pseudomesenteroides TC49 as a D-lactic acid producing strain using rice as a raw material. 2020-06-10T07:12:27Z 2020-06-10T07:12:27Z 2020 Chiang Mai Journal of Science 47,3 (May 2020), p.403-417 2465-3845 https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=10913 http://cmuir.cmu.ac.th/jspui/handle/6653943832/68737 Eng Faculty of Science, Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
continent Asia
country Thailand
Thailand
content_provider Chiang Mai University Library
collection CMU Intellectual Repository
language English
topic Narumol Thongwai - Department of Biology
Faculty of Science
Chiang Mai University
spellingShingle Narumol Thongwai - Department of Biology
Faculty of Science
Chiang Mai University
Tasneem Chemama
Chayakorn Pumas
Yingmanee Tragoolpua
Narumol Thongwai
Feasibility Study for D-Lactic Acid Production from Thai Rice by Leuconostoc pseudomesenteroides TC49 and D-Lactic Acid Purification
description Chiang Mai Journal of Science
author Tasneem Chemama
Chayakorn Pumas
Yingmanee Tragoolpua
Narumol Thongwai
author_facet Tasneem Chemama
Chayakorn Pumas
Yingmanee Tragoolpua
Narumol Thongwai
author_sort Tasneem Chemama
title Feasibility Study for D-Lactic Acid Production from Thai Rice by Leuconostoc pseudomesenteroides TC49 and D-Lactic Acid Purification
title_short Feasibility Study for D-Lactic Acid Production from Thai Rice by Leuconostoc pseudomesenteroides TC49 and D-Lactic Acid Purification
title_full Feasibility Study for D-Lactic Acid Production from Thai Rice by Leuconostoc pseudomesenteroides TC49 and D-Lactic Acid Purification
title_fullStr Feasibility Study for D-Lactic Acid Production from Thai Rice by Leuconostoc pseudomesenteroides TC49 and D-Lactic Acid Purification
title_full_unstemmed Feasibility Study for D-Lactic Acid Production from Thai Rice by Leuconostoc pseudomesenteroides TC49 and D-Lactic Acid Purification
title_sort feasibility study for d-lactic acid production from thai rice by leuconostoc pseudomesenteroides tc49 and d-lactic acid purification
publisher Faculty of Science, Chiang Mai University
publishDate 2020
url https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=10913
http://cmuir.cmu.ac.th/jspui/handle/6653943832/68737
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