A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model

Coomassie brilliant blue (CBB) heated by microwave was used as a staining dye for measuring gelatinolytic activity. The quantity of gelatin remaining after incubation with bacterial collagenase was determined using the heated CBB, resulting in visible blue pellets. Dimethyl sulfoxide was added to di...

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Main Authors:	Osathanunkul M., Buddhachat K., Chomdej S.
格式:	Article
語言:	English
出版:	2014
在線閱讀:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84870695160&partnerID=40&md5=2c294b2515205469c8724e9afe3312c1 http://cmuir.cmu.ac.th/handle/6653943832/6985
標簽:	添加標簽沒有標簽, 成為第一個標記此記錄!
機構:	Chiang Mai University
語言:	English

id	th-cmuir.6653943832-6985
record_format	dspace
spelling	th-cmuir.6653943832-69852014-08-30T03:51:27Z A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model Osathanunkul M. Buddhachat K. Chomdej S. Coomassie brilliant blue (CBB) heated by microwave was used as a staining dye for measuring gelatinolytic activity. The quantity of gelatin remaining after incubation with bacterial collagenase was determined using the heated CBB, resulting in visible blue pellets. Dimethyl sulfoxide was added to dissolve the dye and measurement of the absorbance at 600 nm was done to detect the level of gelatin (up to 10 μg), with the limit of detection for the amount of collagenase at 50 ng. This approach is rapid, simple, and economic for the purpose of screening for pharmaceutical agents that possess inhibitory activity on collagenase. © 2012 Elsevier Inc. All rights reserved. 2014-08-30T03:51:27Z 2014-08-30T03:51:27Z 2013 Article 00032697 10.1016/j.ab.2012.09.036 23072981 ANBCA http://www.scopus.com/inward/record.url?eid=2-s2.0-84870695160&partnerID=40&md5=2c294b2515205469c8724e9afe3312c1 http://cmuir.cmu.ac.th/handle/6653943832/6985 English
institution	Chiang Mai University
building	Chiang Mai University Library
country	Thailand
collection	CMU Intellectual Repository
language	English
description	Coomassie brilliant blue (CBB) heated by microwave was used as a staining dye for measuring gelatinolytic activity. The quantity of gelatin remaining after incubation with bacterial collagenase was determined using the heated CBB, resulting in visible blue pellets. Dimethyl sulfoxide was added to dissolve the dye and measurement of the absorbance at 600 nm was done to detect the level of gelatin (up to 10 μg), with the limit of detection for the amount of collagenase at 50 ng. This approach is rapid, simple, and economic for the purpose of screening for pharmaceutical agents that possess inhibitory activity on collagenase. © 2012 Elsevier Inc. All rights reserved.
format	Article
author	Osathanunkul M. Buddhachat K. Chomdej S.
spellingShingle	Osathanunkul M. Buddhachat K. Chomdej S. A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
author_facet	Osathanunkul M. Buddhachat K. Chomdej S.
author_sort	Osathanunkul M.
title	A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
title_short	A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
title_full	A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
title_fullStr	A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
title_full_unstemmed	A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
title_sort	modified colorimetric method of gelatinolytic assay using bacterial collagenase type ii as a model
publishDate	2014
url	http://www.scopus.com/inward/record.url?eid=2-s2.0-84870695160&partnerID=40&md5=2c294b2515205469c8724e9afe3312c1 http://cmuir.cmu.ac.th/handle/6653943832/6985
_version_	1681420716140920832

A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model

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