A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
Coomassie brilliant blue (CBB) heated by microwave was used as a staining dye for measuring gelatinolytic activity. The quantity of gelatin remaining after incubation with bacterial collagenase was determined using the heated CBB, resulting in visible blue pellets. Dimethyl sulfoxide was added to di...
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| Main Authors: | , , |
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| 格式: | Article |
| 語言: | English |
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2014
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| 在線閱讀: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84870695160&partnerID=40&md5=2c294b2515205469c8724e9afe3312c1 http://cmuir.cmu.ac.th/handle/6653943832/6985 |
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