Feasibility study for D-lactic acid production from thai rice by Leuconostoc pseudomesenteroides TC49 and D-lactic acid purification
© 2020, Chiang Mai University. All rights reserved. Two enantiomers of lactic acid, D- and L-forms, are naturally produced by lactic acid bacteria. Generally, L-lactic acid is more abundant than D-lactic acid. Both forms have been importantly used as monomers in bio-plastic industry. In this study,...
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| Main Authors: | , , , |
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| Format: | Journal |
| Published: |
2020
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| Subjects: | |
| Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85084513129&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/70237 |
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| Institution: | Chiang Mai University |
| Summary: | © 2020, Chiang Mai University. All rights reserved. Two enantiomers of lactic acid, D- and L-forms, are naturally produced by lactic acid bacteria. Generally, L-lactic acid is more abundant than D-lactic acid. Both forms have been importantly used as monomers in bio-plastic industry. In this study, total of 251 lactic acid bacterial strains were isolated and screened from samples collected in southern and northern Thailand. Among these bacteria, only 7 isolates produced D-lactic acid with optical purity ranging from 96.2 to 99.7%. The isolate TC49, identified as Leuconostoc pseudomesenteroides, produced the highest D-lactic acid and was selected for further investigation. When Thai broken rice was used as a raw material for D-lactic acid fermentation by the selected strain, D-lactic acid yield and productivity were 93 ± 0.03 g/l and 1.29 g/l/h, respectively, under the optimal conditions. Upon further recovery and purification by the ultrasonic extraction process, D-lactic acid obtained was 99% optical purity and was structurally examined by 1H-NMR determination. The results indicated the high potential of Leu. pseudomesenteroides TC49 as a D-lactic acid producing strain using rice as a raw material. |
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