Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells

The aim of this study was to investigate the inhibitory mechanism of pure curcumin on WT1 expression in leukemic K562 cells. Pure curcumin suppressed WT1 expression, independent of effects on protein degradation or WT1 mRNA stability. Chromatin immunoprecipitation and reporter gene assays indicate t...

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Main Authors:	Semsri S., Krig S.R., Kotelawala L., Sweeney C.A., Anuchapreeda S.
Format:	Article
Language:	English
Published:	2014
Online Access:	http://www.scopus.com/inward/record.url?eid=2-s2.0-79960383409&partnerID=40&md5=6526c62524f779d17f4e27ab8c1cd4ae http://www.ncbi.nlm.nih.gov/pubmed/21658388 http://cmuir.cmu.ac.th/handle/6653943832/754
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spelling	th-cmuir.6653943832-7542014-08-29T09:02:04Z Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells Semsri S. Krig S.R. Kotelawala L. Sweeney C.A. Anuchapreeda S. The aim of this study was to investigate the inhibitory mechanism of pure curcumin on WT1 expression in leukemic K562 cells. Pure curcumin suppressed WT1 expression, independent of effects on protein degradation or WT1 mRNA stability. Chromatin immunoprecipitation and reporter gene assays indicate that pure curcumin treatment attenuates WT1 auto-regulation. Interestingly, PKCα inhibition mimicks the repressive effects of pure curcumin in K562 cells. Conversely, myristoylated PKCα over-expression increased WT1 expression and reversed the inhibitory effect of pure curcumin. Our study indicates that pure curcumin attenuates WT1 auto-regulatory function through inhibition of PKCα signaling in K562 cells. © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. 2014-08-29T09:02:04Z 2014-08-29T09:02:04Z 2011 Article 145793 10.1016/j.febslet.2011.05.043 21658388 FEBLA http://www.scopus.com/inward/record.url?eid=2-s2.0-79960383409&partnerID=40&md5=6526c62524f779d17f4e27ab8c1cd4ae http://www.ncbi.nlm.nih.gov/pubmed/21658388 http://cmuir.cmu.ac.th/handle/6653943832/754 English
institution	Chiang Mai University
building	Chiang Mai University Library
country	Thailand
collection	CMU Intellectual Repository
language	English
description	The aim of this study was to investigate the inhibitory mechanism of pure curcumin on WT1 expression in leukemic K562 cells. Pure curcumin suppressed WT1 expression, independent of effects on protein degradation or WT1 mRNA stability. Chromatin immunoprecipitation and reporter gene assays indicate that pure curcumin treatment attenuates WT1 auto-regulation. Interestingly, PKCα inhibition mimicks the repressive effects of pure curcumin in K562 cells. Conversely, myristoylated PKCα over-expression increased WT1 expression and reversed the inhibitory effect of pure curcumin. Our study indicates that pure curcumin attenuates WT1 auto-regulatory function through inhibition of PKCα signaling in K562 cells. © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
format	Article
author	Semsri S. Krig S.R. Kotelawala L. Sweeney C.A. Anuchapreeda S.
spellingShingle	Semsri S. Krig S.R. Kotelawala L. Sweeney C.A. Anuchapreeda S. Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells
author_facet	Semsri S. Krig S.R. Kotelawala L. Sweeney C.A. Anuchapreeda S.
author_sort	Semsri S.
title	Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells
title_short	Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells
title_full	Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells
title_fullStr	Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells
title_full_unstemmed	Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells
title_sort	inhibitory mechanism of pure curcumin on wilms' tumor 1 (wt1) gene expression through the pkcα signaling pathway in leukemic k562 cells
publishDate	2014
url	http://www.scopus.com/inward/record.url?eid=2-s2.0-79960383409&partnerID=40&md5=6526c62524f779d17f4e27ab8c1cd4ae http://www.ncbi.nlm.nih.gov/pubmed/21658388 http://cmuir.cmu.ac.th/handle/6653943832/754
_version_	1681419542580953088

Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells

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